连续切片三维重构技术在细胞超微结构研究中的应用  

Application of serial section 3D reconstruction technology in the study of cell ultrastructure

作　　者：王博 戴灵豪 王林燕 关旸 徐纯依 WANG Bo;DAI Ling-hao;WANG Lin-yan;GUAN Yang;XU Chun-yi(Academy of Chinese Medical Sciences,Zhejiang Chinese Medical University,Hangzhou Zhejiang 310053,China)

机构地区：[1]浙江中医药大学中医药科学院,浙江杭州310053

出　　处：《电子显微学报》2022年第1期92-97,共6页Journal of Chinese Electron Microscopy Society

基　　金：浙江省基础公益研究项目(No.LGC19C050001);浙江中医药大学校级基金(No.2019ZY28).

摘　　要：目的:探究三维重构技术在细胞超微结构研究中的重要性。方法:收集Hela细胞,样品经OTO制样方法处理后进行连续切片,并通过扫描电镜收集序列切片背散射电子信号。通过Amira软件对图片集进行对中、分割处理及三维展示。结果:获得细胞三维重构图,对线粒体长度、宽度和厚度进行了测定和统计,着重对线粒体内质网偶联三维结构进行了观察。结论:细胞三维可视化较二维图像能全面客观的展示超微结构,而连续切片三维重构技术在三维可视化研究中具有重要意义。Objective:To explore the importance of 3 D reconstruction technology in cell microstructure research. Methods:Hela cells were collected, the sample was processed by OTO method for serial sections, and the back scattering electronic signals on the sequence sections’ were collected by the scanning electron microscope. The picture collection is aligned, segmented and the 3 D reconstruction was performed with Amira software. Results:The three-dimensional reconstruction of the cell was obtained, the mitochondrial length, width and thickness were measured and counted, and the three-dimensional structure of the mitochondria-associated endoplasmic reticulum membranes was observed. Conclusion:The 3 D visualization can show ultra-microstructure comprehensively and objectively compared to that in two-dimensional image, and the Serial Section 3 D reconstruction technology is of great significance in the study of 3 D visualization.

关 键 词：连续切片 三维重构 细胞 超微结构 

分 类 号：Q248[生物学—细胞生物学] TG115.215.3[金属学及工艺—物理冶金]