模拟干旱胁迫下褪黑素和表油菜素内酯对沟叶结缕草长期继代培养愈伤组织再生的影响  

Effects of melatonin and epibrassinolide on the regeneration of longterm subcultured callus of Zoysia matrella(L.) Merr. under simulated drought stress

作　　者：周认 蔡宇 林恬逸 柴明良[1] ZHOU Ren;CAI Yu;LIN Tianyi;CHAI Mingliang(College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学农业与生物技术学院,杭州310058

出　　处：《浙江大学学报（农业与生命科学版）》2022年第1期36-44,共9页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金(30771522,31672195)。

摘　　要：本试验探究了外源褪黑素(melatonin, MEL)和表油菜素内酯(epibrassinolide, EBL)对继代培养17年的沟叶结缕草[Zoysia matrella (L.) Merr.]愈伤组织在干旱胁迫下再生的影响,旨在筛选干旱胁迫下提高长期继代培养的愈伤组织再生率的最优浓度,并获得耐旱性强的再生植株。结果表明:0.20 mmol/L MEL能显著促进沟叶结缕草愈伤组织再生,再生率为61.1%。干旱胁迫显著抑制了愈伤组织的再生,随着聚乙二醇(polyethylene glycol, PEG)浓度的增加再生率降低,5%、10%PEG-6000胁迫下可获得耐旱性强的再生植株。在5%~15%PEG-6000胁迫下,添加0.05~0.20 mmol/L MEL或0.05~0.50μmol/L EBL对愈伤组织再生具有恢复作用;在10%PEG-6000胁迫下,0.20 mmol/L MEL是促进干旱胁迫下愈伤组织再生的最佳浓度,再生率为55.6%;MEL和EBL浓度过高则抑制再生。此外,在5%、10%PEG-6000胁迫下添加0.05~0.20 mmol/L MEL时,愈伤组织过氧化氢酶(catalase,CAT)活性显著升高;添加0.05~0.10μmol/L EBL时,愈伤组织过氧化物酶(peroxidase, POD)、超氧化物歧化酶(superoxide dismutase, SOD)活性显著升高,2种外源激素诱发沟叶结缕草再生过程中不同抗氧化酶的响应。由此可见,0.05~0.20 mmol/L MEL或0.05~0.10μmol/L EBL对其再生能力具有显著的提升作用。本研究为提高沟叶结缕草长期继代的愈伤组织再生能力以及耐旱植株的筛选提供了新思路。In this study, we explored the effects of exogenous melatonin(MEL) and epibrassinolide(EBL) on the regeneration of callus subcultured for 17 years of Zoysia matrella(L.) Merr. under simulated drought stress,aiming to select the optimal MEL or EBL concentrations that improved the regeneration capacity of callus, and obtain plants with strong drought resistance. The results showed that 0.20 mmol/L MEL markedly promoted the regeneration of callus, and the regeneration rate was 61.1%. Different degrees of drought stress significantly affected the regeneration capacity of callus, and the regeneration rate decreased as polyethylene glycol(PEG)concentration increased. Under 5% and 10% PEG-6000 stress, the regeneration plants of Z. matrella with strong drought tolerance could be obtained. Under 5%-15% PEG-6000 stress, addition of 0.05-0.20 mmol/L MEL or0.05-0.50 μmol/L EBL promoted the recovery of callus regeneration. Under 10% PEG-6000 stress, 0.20 mmol/L MEL was the best treatment to promote the callus regeneration, and the regeneration rate was as high as 55.6%.High concentrations of MEL and EBL inhibited callus regeneration rates. Moreover, when adding 0.05-0.20 mmol/L MEL under 5% or 10% PEG stress, catalase activity of callus increased markedly;when adding 0.05-0.10 μmol/L EBL, peroxidase, superoxide dismutase activities of callus significantly increased, and the two exogenous hormones evoked response of different antioxidant enzymes during the regeneration of Z. matrella callus. It can be seen that,0.05-0.20 mmol/L MEL or 0.05-0.10 μmol/L EBL notably promote the regeneration capacity of Z. matrella callus.This study provides new pathways for improving the regeneration capacity of callus in long-term subculture and drought-resistant selection of Z. matrella.

关 键 词：沟叶结缕草 干旱胁迫 褪黑素 表油菜素内酯 长期继代的愈伤组织 抗氧化酶活性 

分 类 号：S688.4[农业科学—观赏园艺]