异牡荆素对非小细胞性肺癌细胞自我更新和凋亡的影响  

Effect of isovitexin on self-renewal and apoptosis in non-small cell lung cancer cells

作　　者：赵富周[1] 丁成智[2] 李晓明[1] ZHAO Fu-zhou;DING Cheng-zhi;LI Xiao-ming(Department of General Thoracic Surgery,The First Affiliated Hospital of Nanyang Medical College,Nanyang 473000,Henan Province,China;Department of General Thoracic Surgery,Henan Provincial Chest Hospital,Zhengzhou 450000,Henan Province,China)

机构地区：[1]河南省南阳医学高等专科学校第一附属医院胸外科,河南南阳473000 [2]河南省胸科医院胸外科,河南郑州450000

出　　处：《中国临床药理学杂志》2022年第3期219-223,共5页The Chinese Journal of Clinical Pharmacology

基　　金：河南省医学科技攻关计划基金资助项目(2018020555)。

摘　　要：目的研究异牡荆素(ISO)对非小细胞性肺癌(NSCLC)细胞的影响和潜在的机制。方法将A549和H1650细胞分别分为空白组、低剂量实验组(4μmol·L^(-1) ISO)和高剂量实验组(16μmol·L^(-1) ISO)。用噻唑蓝法检测NSCLC细胞活性,用肿瘤球形成实验检测NSCLC细胞的细胞球形成率,用Western blot法检测NSCLC细胞凋亡、自我更新、无翅型MMTV整合位点家族/β-连环蛋白(Wnt/β-catenin)信号通路相关蛋白的表达水平。结果与空白组比较,低、高剂量实验组中A549和H1650细胞在24,48和72 h的细胞活性均显著降低。低、高剂量实验组和空白组中A549细胞的细胞球形成率分别为(4.18±0.45)%,(2.01±0.67)%和(6.02±0.57)%,切割的半胱氨酸蛋白酶-3相对表达量分别为0.24±0.08,1.25±0.13和0.06±0.07,SRY相关高迁移率族盒蛋白-2相对表达量分别为0.49±0.04,0.25±0.03和1.00±0.09,Kruppel样因子4相对表达量分别为0.68±0.04,0.44±0.03和1.01±0.06,Wnt1相对表达量分别为0.63±0.06,0.28±0.04和1.00±0.06,β-catenin相对表达量分别为0.41±0.05,0.22±0.03和1.01±0.09;与空白组比较,低、高剂量实验组中A549细胞的上述指标的差异均有统计学意义(P<0.05,P<0.01)。上述3组中H1650细胞的上述指标也呈现一致的现象。结论ISO可能通过抑制Wnt/β-catenin信号通路来抑制NSCLC细胞活性和自我更新,并促进其凋亡。Objective To investigate the effect of isovitexin(ISO)on non-small cell lung cancer(NSCLC)cells and its potential mechanism.Methods A549 and H1650 cells were divided into blank group,experimental-L group(4μmol·L^(-1) ISO)and experimental-H group(16μmol·L^(-1) ISO).Cell viability in NSCLC cells was measured by methyl thiazolyl tetrazolium assay.Sphere-formation efficiency of NSCLC cells was studied by tumorsphere formation assay.Expressions of apoptosis,self-renewal and wingless type MMTV integration site family/β-catenin(Wnt/β-catenin)signaling pathway-related proteins in NSCLC cells were detected by Western blot.Results Compared with blank group,the cell viabilities of A549 and H1650 cells in experimental-L and experimental-H groups were significantly decreased at 24,48 and 72 h.The sphere-formation efficiencies of A549 cells in experimental-L and experimental-H groups and blank group were(4.18±0.45)%,(2.01±0.67)%and(6.02±0.57)%,the relative expression levels of cleaved cysteinyl aspartate specific proteinase-3 were 0.24±0.08,1.25±0.13 and 0.06±0.07,the relative expression levels of SRY-related high-mobility-group(HMG)-box protein-2 were 0.49±0.04,0.25±0.03 and 1.00±0.09,the relative expression levels of Kruppel-like factor-4 were 0.68±0.04,0.44±0.03 and 1.01±0.06,the relative expression levels of Wnt1 were 0.63±0.06,0.28±0.04 and 1.00±0.06,the relative expression levels ofβ-catenin were 0.41±0.05,0.22±0.03 and 1.01±0.09.Compared with blank group,there were statistically significant differences in the above indexes of A549 cells in experimental-L and experimental-H groups(P<0.05,P<0.01).The above indexes of H1650 cells in the above 3 groups also showed the same phenomenon.Conclusion ISO inhibits cell viability and self-renewal and promotes apoptosis in NSCLC cells by inhibiting Wnt/β-catenin signaling pathway.

关 键 词：异牡荆素 非小细胞性肺癌 无翅型MMTV整合位点家族/β-连环蛋白信号通路 自我更新 凋亡 

分 类 号：R965[医药卫生—药理学]