白芨内生菌Ilyonectria robusta的化学成分研究  

Chemical investigation on the endophytic fungus Ilyonectria robusta from Bletilla sfriata

作　　者：王献 覃晓夏 刘吉开[1] WANG Xian;QIN Xiaoxia;LIU Jikai(School of Pharmaceutical Sciences,South-Central Minzu University,Wuhan 430074,China)

机构地区：[1]中南民族大学药学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2022年第2期169-173,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(22177138)。

摘　　要：采用硅胶、Sephadex LH-20、中压制备以及HPLC等色谱分离方法对一株白芨内生真菌Ilyonectria robusta进行化学成分研究,分离得到2个新化合物和4个已知化合物.通过UV、IR、NMR、MS等多种波谱手段并结合文献数据对比,鉴定其结构分别为:butyl((S)-5-oxopyrrolidine-2-carbonyl)-D-leucinate(1)、butyl((S)-5-oxopyrrolidine-2-carbonyl)-D-phenylalaninate(2)、4-hydroxy-17R-methylincistero(3)、polyporenic acid C(4)、perlolyrine(5)和4-(9H-βCarbolin-1-yl)-4-oxobut-2-enoic acid methyl ester(6).以上化合物均首次从该菌中分离得到,化合物1和2为新化合物.采用MTT法检测了化合物1和2对人类乳腺癌细胞MCF-7的细胞毒活性,化合物1对MCF-7具有一定的抑制作用,IC_(50)为36.2µM.Column chromatography over silica gel,Sephadex LH-20,MPLC,and HPLC led to the isolation of six compounds from an endophytic fungus Ilyonectria robusta characterized from Chinese medicinal plant Bletilla striata.Their structures were established by means of spectroscopic methods including UV,IR,NMR and MS.Compounds 1 and 2 were previously undescribed.All compounds were identified as butyl((S)-5-oxopyrrolidine-2-carbonyl)-D-leucinate(1),butyl((S)-5-oxopyrrolidine-2-carbonyl)-D-phenylalaninate(2),4-hydroxy-17R-methylincistero(3),polyporenic acid C(4),perlolyrine(5)and 4-(9H-β-Carbolin-1-yl)-4-oxobut-2-enoic acid methyl ester(6).All compounds are isolated from this fungus for the first time.The MTT method was used to evaluate the cytotoxic activity of compounds 1 and 2 on human tumor cell lines MCF-7.Compound 1 exhibited certain cytotoxicity on MCF-7 with an IC_(50) values of 36.2µM.

关 键 词：白芨内生菌 Ilyonectria robusta 化学成分 结构鉴定 细胞毒性 

分 类 号：O657.61[理学—分析化学] R284.1[理学—化学]