流行性乙型脑炎病毒GI型毒株SD12在C57BL/6小鼠体内的动态分布研究  

Dynamic Distribution of Gene Type Japanese Encephalitis Virus Loads in Infected C57BL/6 Mice

作　　者：王欣[1,2] 郭爽[2] 张俊杰 庞琳琳 李蓓蓓 刘珂[2] 邵东华[2] 邱亚峰[2] 马志永[2] 钟登科[3] 李鹏[1] 魏建超[2] WANG Xin;GUO Shuang;ZHANG Junjie;PANG Linlin;LI Beibei;LIU Ke;SHAO Donghua;QIU Yafeng;MA Zhiyong;ZHONG Dengke;LI peng;WEI Jianchao(Yangtze University,Jingzhou 434000,China;Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;Shanghai Vocational and Technical College of Agriculture and Forestry,Shanghai 201600,China)

机构地区：[1]长江大学,荆州434000 [2]中国农业科学院上海兽医研究所,上海200241 [3]上海农林职业技术学院,上海201600

出　　处：《中国动物传染病学报》2022年第1期150-156,共7页Chinese Journal of Animal Infectious Diseases

基　　金：上海市自然基金项目面上项目(19ZR1469000);中央级公益性科研院所基本科研业务费(2019JB10);上海农林职业技术学院科研项目(KY1-0000-17-09)。

摘　　要：为了解流行性乙型脑炎病毒基因I型毒株SD12在感染C57BL/6小鼠体内的病毒分布,应用qRT-PCR方法检测该毒株在小鼠体内各组织的动态分布情况。结果显示腹腔感染组中2 dpi仅能在心脏和脾脏检测出病毒核酸,5~7 dpi能够在心脏、肝脏、脑部、盲肠和扁桃体检测到病毒核酸。血脑屏障受损的腹腔感染组中最早2 dpi在肠系膜淋巴结中检测到病毒核酸,3 dpi基本能在各组织中检测到病毒核酸,5~7 dpi在大脑中检测到大量病毒核酸。所有对照组没有检测到病毒阳性核酸。本研究表明基因I型流行性乙型脑炎病毒感染血脑屏障受损的C57BL/6小鼠后能迅速入侵各组织脏器,主要浸染肝脏、肺脏、肾脏、盲肠和大脑。拥有完整血脑屏障的小鼠对病毒具有一定抵抗能力,病毒在各组织中的含量相对比较低。To investigate the distribution of the viral loads in C57BL/6 mice infected with Japanese encephalitis virus(JEV)type I strain SD12,various tissues were collected and tested using qRT-PCR.The results showed that the viral nucleic acids were detected in spleens and hearts at 2 dpi and in tonsils,hearts,livers,brains and ceca at 5~7 dpi for the intraperitoneally infected group.In the abdominally infected group with previously impaired blood-brain barriers,viral nucleic acids were detected in the mesenteric lymph nodes at 2 dpi,in all tissues at 3 dpi,and in brains at 5-7 dpi.No viral nucleic acid was detected in all control groups.This study showed that genotype I JEV rapidly invaded all tissues of C57BL/6 mice with blood-brain barriers previously impaired,causing heavy viral loads detected in livers,lungs,kidneys,ceca and brains.On the contrast,a relatively low viral loads were detected,suggesting that mice with intact blood-brain barriers had some resistance to JEV infection.

关 键 词：流行性乙型脑炎病毒 血脑屏障 QRT-PCR 动态分布 

分 类 号：S852.659.6[农业科学—基础兽医学]