鸡白痢、鸡伤寒沙门菌分子分型方法的验证  

Verifi cation of Molecular Typing Methods for Salmonella Pullorum and Salmonella Gallinarum

作　　者：龚建森[1] 徐敬潇 张笛[1] 祁克宗[2] 徐步[1] 窦新红[1] 董永毅[3] GONG Jiansen;XU Jingxiao;ZHANG Di;QI Kezong;XU Bu;DOU Xinhong;DONG Yongyi(Jiangsu Institute of Poultry Sciences,Yangzhou 225125,China;Key Laboratory of Veterinary Pathology and Disease Control and Prevention of Anhui Province,Anhui Agricultural University,Hefei 230036,China;Jiangsu Animal Disease Prevention and Control Center,Nanjing 210036,China)

机构地区：[1]江苏省家禽科学研究所,扬州225125 [2]安徽农业大学兽医病理生物学与疫病防控安徽省重点实验室,合肥230036 [3]江苏省动物疫病预防控制中心,南京210036

出　　处：《中国动物传染病学报》2022年第1期166-172,共7页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然科学基金(31402200,31772758);江苏现代农业产业技术体系建设项目(JATS〔2021〕357、JATS〔2021〕398)。

摘　　要：为验证鸡白痢、鸡伤寒沙门菌分子分型方法的准确性,本研究以3株鸡白痢、鸡伤寒沙门菌国际参考菌株和306株国内分离株为研究对象,采用5种已知的分子分型方法开展验证性实验。结果发现,基于特定基因可变区的2种分型方法具有良好的特异性,其检测结果与生化分型结果一致,而3种基于特定基因单核苷酸多态性的分型方法则出现假阳性或假阴性的结果。上述结果表明,鸡白痢、鸡伤寒沙门菌分子分型方法的准确性仍需进一步验证。The aim of this study was to verify the accuracy of molecular typing methods for Salmonella Pullorum and Salmonella Gallinarum.In this study,three international reference strains and 306 Chinese isolates of Salmonella Pullorum and Salmonella Gallinarum were used for verifying fi ve known molecular typing methods.The results showed that two genotyping methods based on variable regions of specifi c genes had good specifi city and their detection results were consistent with biochemical typing results while the other three genotyping methods based on single nucleotide polymorphism of specifi c genes produced false positive or false negative results.Therefore,the molecular typing methods for Salmonella Pullorum and Salmonella Gallinarum still needs to be further verifi ed for their accuracy.

关 键 词：鸡白痢沙门菌 鸡伤寒沙门菌 单核苷酸多态性 生化鉴定 分子分型 

分 类 号：S852.612[农业科学—基础兽医学]