黑萝卜胞质类型的分子鉴定与育种利用  

Molecular identification of cytoplasm types of black radish(Raphanus sativus L.var.niger)and the breeding utilization

作　　者：李晓梅[1,2,3] 杨峰 雍晓平[1] 冉茂林 LI Xiaomei;YANG Feng;YONG Xiaoping;RAN Maolin(Rice and Sorghum Research Institute,Sichuan Academy of Agricultural Sciences,Deyang 618000,Sichuan,China;College of Horticulture,Sichuan Agricultural University,Chengdu 611130,Sichuan,China;Vegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan,Chengdu 610300,Sichuan,China)

机构地区：[1]四川省农业科学院水稻高粱研究所,四川德阳618000 [2]四川农业大学园艺学院,成都611130 [3]蔬菜种质与品种改良四川省重点实验室,成都610300

出　　处：《中国瓜菜》2022年第2期7-11,共5页China Cucurbits And Vegetables

基　　金：四川省科技计划应用基础研究项目(2018JY0632);四川省财政能力提升工程专项资金项目(2019QNJJ-021);四川省农业科学院青年基金项目(2019QNJJ-021)。

摘　　要：萝卜胞质类型鉴定是杂种优势利用的前提。以NWB/DCGMS CMS及Ogura CMS两个萝卜细胞质雄性不育特异基因设计引物,对3份黑萝卜材料进行胞质类型鉴定。结果表明,3份可育黑萝卜资源均含有NWB/DCGMS CMS不育主控基因orf463,利用3份黑萝卜为母本与其他萝卜构建的F2代遗传群体表现出育性分离现象,根据育性分离比发现黑萝卜R3、R4含2对恢复基因,黑萝卜R5含3对恢复基因。同时,利用黑萝卜含有NWB/DCGMS CMS线粒体不育主控基因及细胞核恢复基因的特性,进行了不育系及恢复系的创制选育,为萝卜NWB/DCGMS CMS杂交品种的选育利用提供了技术支撑。The identification of radish cytoplasm is the prerequisite for the utilization of heterosis.We used NWB/DCGMS and Ogura CMS gene specific primers to identify the cytoplasm types of three black radish lines.The result showed that all three fertile black radishes had NWB/DCGMS CMS major gene orf463.The segregation of F2’s using the three black radishes as female parents and other radish as male parents suggest that black radish R3,R4 carried 2 pairs of restorer genes,and black radish R5 carried 3 pairs of restorer genes.Taking advantage of black radish having both NWB/DCGMS CMS mitochondrial sterility gene and nuclear restorer gene,we carried out the breeding of sterile lines and restorer lines to provide resources for breeding and utilization of NWB/DCGMS CMS hybrids.

关 键 词：黑萝卜 细胞质雄性不育 育种利用 

分 类 号：S631.1[农业科学—蔬菜学]