锌处理对霍山石斛花瓣挥发油、非挥发性有机物及超微结构的影响  

Determination of volatile oil and non-volatile organic compounds contents and ultrastructure changes in the petals of Dendrobium huoshanense upon Zn application

作　　者：朱旺生[1] 戴军[1] 陈乃东[1] 王佳宏[2] ZHU Wangsheng;DAI Jun;CHEN Naidong;WANG Jiahong(Engineering Technology Research Center for Plant Cell of Anhui Province,West Anhui University,Lu’an 237012,China;Department of Food Science and Technology,College of Light Industry and Food Engineering,Nanjing Forestry University,Nanjing 210037,China)

机构地区：[1]皖西学院植物细胞安徽省工程技术研究中心,六安237012 [2]南京林业大学轻工与食品工程学院食品科学与技术系,南京210037

出　　处：《中国生态农业学报（中英文）》2022年第3期419-430,共12页Chinese Journal of Eco-Agriculture

基　　金：supported by the National Natural Science Foundation of China(81573536);the Key Project of the Natural Science of Universities of Anhui Province(KJ2018A0421).

摘　　要：霍山石斛花瓣具有宜人的香气和保健价值,具有开发功能性食品的潜力。为获得用于功能性食品生产的优质原料,在大田栽培条件下提高霍山石斛花瓣的食品品质性状越来越受到人们关注。该研究主要关注Zn施用对霍山石斛花瓣中挥发油和非挥发性有机物的影响,并从花瓣超微结构变化视角探讨其机制。采用气相色谱-质谱联用技术(GC-MS)分析挥发油成分,化学分析法测定非挥发性有机物(可溶性糖、游离氨基酸和酚类化合物)含量,透射电镜观察超微结构变化。结果表明,施Zn提高了霍山石斛花瓣挥发油(主要由萜烯类及其衍生物组成)和非挥发性有机物含量,不同Zn处理对挥发油和非挥发性有机物生产影响差异明显。挥发油、可溶性糖、自由氨基酸及酚类化合物含量最大增幅分别达28.57%、33.53%、28.89%和58.41%。超微结构变化表明,施Zn促进花瓣细胞液泡融合,增加细胞中线粒体、淀粉粒和脂滴的产生。Zn施用能提高霍山石斛花瓣挥发油和非挥发性有机物的含量。超微结构分析能为挥发油和非挥发性有机物生产提供新的解剖学见解。The petals of Dendrobium huoshanense have great potential as functional food because of their pleasant fragrance and health values.To obtain high-quality raw materials for functional food production,more attention is being directed toward improving the food quality traits of D.huoshanense petals under field cultivation conditions.In this study,we investigated the effects of Zn application on the volatile oil and non-volatile organic compounds contents in the petals of D.huoshanense.Furthermore,the mechanism was also studied from the perspective of petal ultrastructural changes.Volatile oil constituents were investigated using gas chromatography-mass spectrometry.The contents of non-volatile organic compounds,including soluble sugar,free amino acids,and phenolic compounds,were investigated using chemical analysis methods.Ultrastructural changes were observed via transmission electron microscopy.The results showed that Zn application increased the yield of volatile oils(primarily terpenes and their derivatives)and non-volatile organic compounds from the petals of D.huoshanense.Different levels of Zn treatments changed the production of the above-mentioned volatiles and non-volatile compounds to varying degrees.The maximum rate of increase in the content of volatile oil,soluble sugars,free amino acids,and phenolic compounds was 28.57%,33.53%,28.89%,and 58.41%,respectively.Ultrastructural changes at the cellular level showed that Zn application promoted vacuole fusion and increased the production of mitochondria,starch grains,and lipid droplets.In conclusion,Zn application improves the production of volatile oils and non-volatile organic compounds.Ultrastructural analysis has provided new anatomical insights into the production of volatile oils and non-volatile organic compounds.

关 键 词：霍山石斛 非挥发物 超微结构 挥发物 Zn水平 

分 类 号：S573[农业科学—作物学]