矾根组培增殖技术研究  

Study on Multiplication Technology of Heuchera micrantha

作　　者：朱志国[1] ZHU Zhi-guo(Department of Landscape Architecture and Horticulture,Wuhu Institute of Technology,Wuhu,Anhui 241003)

机构地区：[1]芜湖职业技术学院园林园艺学院,安徽芜湖241003

出　　处：《安徽农业科学》2022年第4期42-43,47,共3页Journal of Anhui Agricultural Sciences

基　　金：安徽高校省级自然科学重点研究项目基金(KJ2020A0923);芜湖职业技术学院科技创新服务平台培育项目基金(Kjcxpt202007)。

摘　　要：以矾根(Heuchera micrantha)初代培养获得的无菌苗切割成茎段作为外植体,对矾根组织培养增殖技术进行研究。结果表明,采用MS作为基本培养基,通过6-BA和NAA组合进行试验筛选,得到最佳培养基配方是MS+NAA 0.5 mg/L+6-BA 1.0 mg/L,此时矾根增殖系数为9.76;不同培养方式对矾根增殖影响有明显差异,采用0.2%琼脂培养基增殖效果最好;矾根增殖系数与培养时间呈线性关系,培养时间以40 d较好;采用0.2%琼脂培养基对矾根进行培养时,pH为5.0效果最佳。The tissue culture technique was studied by cutting the sterile seedlings obtained from the primary culture of Heuchera micrantha into stem segments as explants.The results showed that MS was used as the basic medium,and the optimal medium for tissue propagation of Heuchera micrantha was MS+NAA 0.5 mg/L+6-BA 1.0 mg/L through the combined test of 6-BA and NAA,in which the reproduction coefficient was 9.76.Different culture methods had obvious differences in the propagation of Heuchera micrantha.Among them,the 0.2%agar medium close to the fluid state had the best proliferation effect.In addition,the propagation of Heuchera micrantha had a linear relationship with the culture time,and the culture time was 40 d;when the culture medium close to the fluid state was used to multiply Heuchera micrantha,the effect was best when the pH value was 5.0.

关 键 词：矾根 组织培养 快速繁殖 增殖 

分 类 号：S682.1[农业科学—观赏园艺]