盆栽小菊组培快繁体系的建立及玻璃化研究  

Establishment of Tissue Culture and Rapid Propagation System of Potted Chrysanthemum and Vitrification Study

作　　者：刘丹 陈天烺 王容 徐春光 吴海峰 赵鹏霞 陈炜 赫文韬 LIU Dan;CHEN Tian-lang;WANG Rong(Zhejiang Hifun Flower Co.,Ltd.,Shaoxing,Zhejiang 312000)

机构地区：[1]浙江海丰花卉有限公司,浙江绍兴312000

出　　处：《安徽农业科学》2022年第4期44-47,共4页Journal of Anhui Agricultural Sciences

基　　金：浙江省重点研发计划项目(2019C02025-3)。

摘　　要：以盆栽小菊“子午线”的花瓣作为外植体,研究不同消毒时间对外植体污染率的影响,以及不同激素浓度对花瓣诱导愈伤组织和不定芽分化的影响;以及对缓解玻璃化苗方法的探讨。结果表明,当使用次氯酸钠处理花瓣时,7 min是最佳处理时间。MS+6-BA 2.0 mg/L+NAA1.0 mg/L+蔗糖30 g/L+琼脂5 g/L是诱导花瓣愈伤组织的最佳培养基,诱导率达98%。不定芽分化最适培养基为MS+6-BA 3.0 mg/L+NAA 0.1 mg/L+蔗糖30 g/L+琼脂5 g/L,分化率达90.30%。培养基MS+6-BA 0.2 mg/L+蔗糖30 g/L+琼脂5 g/L是缓解菊花玻璃化现象的最佳配方,转化为正常苗的转化率最高,可达98.36%。The petals of potted Chrysanthemum“Meridian”were used as explants to study the effects of different disinfection times on explant contamination rate and hormone concentration on petal-induced callus and adventitious bud differentiation.The methods of alleviating vitrification were also discussed.The results showed that when the petals were treated with sodium hypochlorite,7 min was the best treatment time.MS+6-BA 2.0 mg/L+NAA 1.0 mg/L+sucrose 30 g/L+agar 5 g/L was the best medium to induce petal callus,the induction rate was 98%.MS+6-BA 3.0 mg/L+NAA 0.1 mg/L+sucrose 30 g/L+Agar 5 g/L was the optimum medium for adventitious bud differentiation,the differentiation rate was 90.30%.To solve the problem of vitrification,MS+6-BA 0.2 mg/L+sucrose 30 g/L+Agar 5 g/L was the best formula to alleviate the vitrification of Chrysanthemum,and the highest transformation rate was achieved to 98.36%.

关 键 词：花瓣 愈伤组织 玻璃化苗 诱导率 分化率 

分 类 号：S682.1[农业科学—观赏园艺]