差示苯酚-硫酸法结合DNS法测定红芪多糖(HPS)含量  

Differential Phenol-Sulfuric Acid Method Combined with DNS Method to Determine the Content of Hedysarum Polysaccharides(HPS)

作　　者：张小荣 黄钰芳[1,2,3] 何海 赵沙沙 郭玫 邵晶[1,2,3,4] ZHANG Xiao-rong;HUANG Yu-fang;HE Hai(Gansu University of Chinese Medicine,Lanzhou,Gansu 730000;Northwest Collaborative Innovation Center for Traditional Chinese Medicine,Lanzhou,Gansu 730000;Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines of the College of Gansu Province,Gansu University of Chinese Medicine,Lanzhou,Gansu 730000)

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]西北中藏药协同创新中心,甘肃兰州730000 [3]甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000 [4]甘肃省中药药理与毒理学重点实验室,甘肃兰州730000

出　　处：《安徽农业科学》2022年第4期186-189,253,共5页Journal of Anhui Agricultural Sciences

基　　金：兰州市城关区科技计划项目(2020-2-2-2);甘肃省科技厅自然科学基金项目:(17JR5RA163);甘肃省中药制药工艺工程研究中心开放课题(ZYGY202004);中医药公共卫生服务补助专项子课题(2305191901);甘肃省中药药理与毒理学重点实验室开放基金项目:(ZDSYS-KJ-2018-005)。

摘　　要：[目的]优化建立差示苯酚-硫酸法结合DNS法测定红芪多糖含量的方法。[方法]采用单因素试验筛选考察差示苯酚-硫酸法的检测波长、显色剂用量、反应时间等条件;通过线性关系、精密度、稳定性、重复性、加样回收率分别对差示苯酚-硫酸法和DNS法进行方法学考察。[结果]差示苯酚-硫酸法测得的红芪样品中以葡萄糖计的总糖含量平均值为72.49%,DNS法测得样品中以葡萄糖计的还原性单糖含量平均值为8.13%,以葡萄糖计的多糖含量平均为64.36%,RSD均小于4%。[结论]该研究采用所建立的差示苯酚-硫酸法结合DNS法测定了红芪多糖的含量,结果显示此方法较准确稳定、重复性较好,在一定程度上减少游离还原糖及杂质的干扰,降低了由于操作等带来的系统误差,使样品中多糖含量的测定结果更接近真实值,为红芪多糖等中药多糖的含量测定提供更好的参考方法。[Objective]To optimize the establishment of a differential phenol-sulfuric acid method combined with DNS method for the determination of HPS content.[Method]Single factor experiment was used to screen and investigate the detection wavelength,developer dosage,reaction time and other conditions of the differential phenol-sulfuric acid method;the differential phenol was determined by linear relationship,precision,stability,repeatability and sample recovery.Sulfuric acid method and DNS method for methodological investigation.[Result]The average total sugar content in the Hedysari radix sample measured by the differential phenol-sulfuric acid method was 72.49%,and the average reduction monosaccharide content in the sample measured by the DNS method was 8.13%,which was calculated as the glucose.The average content of polysaccharides was 64.36%,and the RSD was less than 4%.[Conclusion]The study uses the established differential phenol-sulfuric acid method combined with the DNS method to determine the HPS content.The results show that this method is more accurate,stable and has good repeatability.It reduces the interference of free reducing sugars and impurities to a certain extent,and reduces the system error caused by operation,etc.,makes the determination result of polysaccharide content in the sample closer to the true value,which will provide a better reference method for the determination of polysaccharide content in traditional Chinese medicine such as HPS.

关 键 词：红芪多糖 差示苯酚-硫酸法 DNS法 含量测定 

分 类 号：R284[医药卫生—中药学]