基于CRISPR/Cas系统的现场核酸检测技术  

An Review on a Technology for Field Detection of Nucleic Acids Based on CRISPR/Cas System

作　　者：蒋静 赵文亮 李文越 陶大刚 潘文雅 徐兵荣 肖箫 谢胜松[4] 蒋羽 Jiang Jing;Zhao Wenliang;Li Wenyue;Tao Dagang;Pan Wenya;Xu Bingrong;Xiao Xiao;Xie Shengsong;Jiang Yu(Technical Center for Animal Plant and Food Inspection and Quarantine of Shanghai Customs,Shanghai 200135,China;Technical Center for Mechanical and Electrical Product Inspection and Testing of Shanghai Customs Districte,Shanghai 201308,China;Shanghai International Travel Health Care Center,Shanghai 201206,China;Huazhong Agricultural University,Wuhan,Hubei 430070,China;Yangshan Customs,Shanghai 201308,China)

机构地区：[1]上海海关动植物与食品检验检疫技术中心,上海200135 [2]上海海关机电产品检测技术中心,上海201308 [3]上海海关国际旅行卫生保健中心,上海201206 [4]华中农业大学,湖北武汉430070 [5]洋山海关,上海201308

出　　处：《中国动物检疫》2022年第3期55-60,共6页China Animal Health Inspection

基　　金：科技部重点研发计划项目(2018YFC1603600,2018YFC1603603);上海海关科研项目(SHK007-2020)。

摘　　要：核酸检测技术在病原鉴定中发挥了至关重要的作用。传统核酸检测主要依赖聚合酶链式反应(polymerase chain reaction,PCR)技术,但该技术难以满足便携式现场核酸检测需求。近年来发明的CRISPR/Cas生物传感技术可实现高灵敏度、高特异性、快速准确的现场核酸检测。本文重点探讨了CRISPR/Cas生物传感技术的最新进展,包括该技术的原理、优缺点以及在现场快速核酸检测中的应用。CRISPR/Cas技术已被证明是非常适合现场核酸检测的新技术,相信未来在核酸检测领域将得到更广泛应用。The technology for detecting nucleic acids plays an important role in pathogen identification.Traditional detection of nucleic acids mainly depends on polymerase chain reaction(PCR)technology that is difficult to meet the needs for portable field detection of nucleic acids,which could be overcome by the CRISPR/Cas biosensor technology developed in recent years due to its high sensitivity,specificity,rapidity and accuracy.In the paper,the latest research progress of CRISPR/Cas biosensor technology was discussed,including its principle,advantages and disadvantages as well as application in field detection for nucleic acids.It was proved that the technology was very suitable for field detection of nucleic acids,which was believed to be widely used in the area in the future.

关 键 词：CRISPR/Cas技术 核酸检测 现场可视化 

分 类 号：S851.3[农业科学—预防兽医学]