紫鸭跖草根组织应答铜胁迫的转录组分析  

Transcriptome Analysis of Response to Heavy Metal Copper Stress in Setcreasea purpurea Root Tissue

作　　者：彭国颖 胡亮 黄超[2] 杨坤[1] 万玮 黄长干[1] PENG Guo-ying;HU Liang;HUANG Chao;YANG Kun;WAN Wei;HUANG Chang-gan(Jiangxi Agricultural University,Nanchang Key Laboratory of Chemical Utilization of Plant Resources,Nanchang 330045;Jiangxi Agricultural University Alumni Office,Nanchang 330045)

机构地区：[1]江西农业大学南昌市植物资源化学利用重点实验室,南昌330045 [2]江西农业大学校友办,南昌330045

出　　处：《生物技术通报》2022年第2期83-94,共12页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31760068)。

摘　　要：紫鸭跖草是一种高耐铜的超积累植物,本研究首次应用RNA-Seq技术对其转录组进行分析。通过全长转录组分析组装了紫鸭跖草耐铜相关候选基因,通过转录组分析,共获得82 471条N50长度为2 299 bp的高质量unigene,为紫鸭跖草的进一步研究提供了丰富的数据。对照组(CK)、300 μmol/L胁迫组(CT1)和1 000 μmol/L胁迫组(CT2)的测序数据已保存在NCBI的SRA数据库中,登录号为SAMN11265427。CT1相比于CK,共有5 028条unigene在根组织中有显著性差异表达,约占全部unigene的6.10%,其中富集上调和下调的基因分别为3 138和1 890条;CT2相比于CT1,根中共有6 813个unigene富集差异表达,占所有unigene的8.26%。其中富集上调和下调的基因分别为2 555和4 258。随机选取10个基因进行qRT-PCR荧光定量分析,结果与Illumina测序数据一致,验证了基因数据差异表达的有效性。上述实验从分子水平上为研究铜胁迫下铜耐受的分子机制提供了理论依据。Setcreasea purpurea is a highly copper(Cu)tolerant and hyperaccumulator plant.It is the first time that RNA-Seq technology was applied to profile the transcriptome of it in this study.Candidate genes related to copper tolerance of S.purpurea were assembled based on de novo transcriptome analysis.A total of 82 471 high-quality unigenes with an N50 length of 2 299 bp were obtained through transcriptome profiling,which provided abundant data for future studies on S.purpurea.The sequencing data of the control group(CK),300 mol/L stress group(CT1),and 1 000 mol/L stress group(CT2)were deposited in the NCBI sequence read archive database under the accession number SAMN11265427.After comparing CT1 with CK,a total of 5 028 unigenes were significantly differentially expressed in the roots,accounting for 6.10% of all unigenes.Among them,the significantly up-regulated unigenes and down-regulated unigenes were 3 138 and 1 890,respectively.After comparing CT2 with CT1,a total of 6 813 unigenes were significantly differentially expressed in the roots,accounting for 8.26% of all unigenes.Among them,the significantly up-regulated unigenes and down-regulated unigenes were 2 555 and 4 258,respectively.The results were consistent with Illumina sequencing data based on the quantitative fluorescence analysis of 10 randomly selected genes by qRT-PCR,which confirmed the validity of differentially expressed gene data.The above experiment provides a theoretical basis for examining the molecular mechanism of copper tolerance under copper stress on the molecular level.

关 键 词：紫鸭跖草 根组织 铜胁迫 转录组 

分 类 号：Q945.78[生物学—植物学]