核糖核酸5'端NAD^(+)帽子修饰研究进展  

Research Progress in NAD^(+)Cap Modification at the 5’ End of RNA

作　　者：董海娇 杨晓玉[3] 莫蓓莘 陈雪梅[4] 崔洁 DONG Hai-jiao;YANG Xiao-yu;MO Bei-xin;CHEN Xue-mei;CUI Jie(College of Life Sciences and Oceanography,Shenzhen University,Shenzhen 518037;Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,College of Optoelectronic Engineering,Shenzhen University,Shenzhen 518060;College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an 271018;Department of Botany and Plant Sciences,Institute of Integrative Genome Biology,University of California,Riverside,CA,92521,USA)

机构地区：[1]深圳大学生命与海洋科学学院,深圳518037 [2]深圳大学光电工程学院光电子器件与系统(教育部/广东省)重点实验室,深圳518060 [3]山东农业大学园艺科学与工程学院,泰安271018 [4]美国加利福尼亚大学河滨分校整合基因组学研究中心/植物科学系,美国加州河滨92521

出　　处：《生物技术通报》2022年第2期245-251,共7页Biotechnology Bulletin

基　　金：国家自然科学基金青年科学基金项目(31801077,32000153);中国博士后科学基金面上资助(2018M640822);深圳市高等院校稳定支持计划面上项目(20200809224536001)。

摘　　要：m7G帽子具有保护RNA不被降解以及招募相关蛋白参与内含子剪切、poly(A)加尾、出核和翻译等功能。一直以来,它被认为是真核生物mRNA所特有的修饰类型。然而近年来,在包括原核生物在内的多个物种中均检测到一种新的RNA 5’端修饰,即核酸代谢物烟酰胺腺嘌呤二核苷酸(nicotinamide adenine dinucleotide,NAD^(+))帽子。目前NAD^(+)修饰RNA(NAD-RNA)的生物学功能研究仍处于起始阶段。本文概述了NAD-RNA的发现及其检测和鉴定技术的发展;探讨了NAD^(+)帽子对RNA的调控功能,以及NAD-RNA脱帽和加帽的影响因素;并进一步推测NAD-RNA在生物的生长、发育和环境响应中发挥的潜在功能。最后,展望了未来NAD-RNA的研究方向和主题。The m7G cap,which has long been considered as a hallmark of eukaryotic mRNAs,protects RNAs from degradation and recruits the machinery for splicing,polyadenylation,nuclear export,and translation.The nucleotide-containing metabolite,nicotinamide adenine dinucleotide(NAD^(+)),has recently been detected at the 5’ end of RNAs in several species including prokaryotes as a new cap.Now research on the biological functions of NAD^(+)-capped RNAs(NAD-RNAs)is still at the initial stage.This review summarizes the discovery of NAD-RNAs as well as the development of its detection and identification technology,discusses the regulatory functions of the NAD^(+)cap on RNA as well as the factors affecting RNA NAD^(+)capping and decapping,and speculates on the potential functions of NAD-RNAs in growth,development and environmental responses.Finally,we propose future research directions and topics in order to garner attention on the outstanding questions regarding the molecular and biological functions NAD-RNAs.

关 键 词：RNA帽子 NAD^(+) NAD-RNA 转录后调控 生物学功能 

分 类 号：Q75[生物学—分子生物学]