血浆mi R-195对早期急性心肌梗死的诊断价值及可能机制的生物信息学分析  

Diagnostic value of plasma miR-195 in early acute myocardial infarction and bioinformatics analysis of possible mechanisms

作　　者：张淑华[1] 陈延宇 刘燕锋[1] 王云霞[1] 姚宇迪 吴志勇[1] ZHANG Shuhua;CHEN Yanyu;LIU Yanfeng;WANG Yunxia;YAO Yudi;WU Zhiyong(Cardiovascular Research Institute,Jiangxi Provincial People's Hospital(The First Affiliated Hospital of Nanchang Medical College),Nanchang 330006,China)

机构地区：[1]江西省人民医院(南昌医学院第一附属医院)心血管病研究所,南昌330006 [2]南昌大学医学院 [3]南昌大学第一附属医院高新医院中心实验室

出　　处：《山东医药》2022年第7期22-25,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81260031);江西省自然科学基金资助项目(20192BAB205007);江西省卫生计生委科技计划项目(20181014)。

摘　　要：目的探讨血浆微小RNA-195(miR-195)对早期急性心肌梗死(AMI)的诊断价值及可能机制的生物信息学分析。方法选取AMI患者100例作为AMI组,同期选择健康志愿者60例作为对照组,抽取静脉血并分离血浆。用固相免疫层析法检测心肌肌钙蛋白I(cTnI),用实时定量PCR法检测血浆miR-195表达;用受试者工作特征曲线评价cTnI、miR-195对AMI的诊断效能;用miRDB和miRWalk数据库预测miR-195的靶基因,并筛选与AMI相关基因,取交集进行基因本体数据库(GO)富集和京都基因与基因组百科全书(KEGG)通路分析。结果 AMI组胸痛发生4 h即可在血浆中检测到miR-195表达,且随胸痛时间延长血浆miR-195表达呈升高趋势,与对照组比较差异有统计学意义(P均<0.05)。miR-195诊断AMI的曲线下面积为0.94,敏感度、特异度分别为90.0%、94.7%;miR-195联合cTnI诊断AMI的曲线下面积、敏感度和特异度分别为0.96、91.0%、93.0%。miR-195的靶基因与AMI相关基因的交集基因多富集于细胞凋亡的负向调控通路。结论血浆miR-195对AMI具有较好的早期诊断效能,其机制可能是miR-195靶基因参与了心肌细胞凋亡的调控。Objective To evaluate the diagnostic value of plasma microRNA-195(miR-195)in early acute myocar⁃dial infarction(AMI)and the bioinformatic analysis of its possible mechanism.Methods A total of 100 AMI patients(AMI group)were enrolled in the study,and 60 healthy volunteers were selected as the control group.Venous blood was collected and plasma was separated.Cardiac troponin I(cTnI)was detected by solid phase immunochromatography and plasma miR-195 was detected by real-time quantitative PCR.Then the diagnostic efficacy of cTnI and miR-195 in AMI was evaluated by receiver operating characteristic(ROC)curve.Meanwhile,miRDB and miRWalk databases were used to predict the target genes of miR-195,and AMI disease-related genes were screened.Whereafter the intersection of miR-195 was used for gene ontology database(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)path⁃way analysis.Results The miR-195 expression was detected in plasma at 4 h after the onset of chest pain in the AMI group,and increased with the prolonging of chest pain;there was statistically significant difference between the AMI group and the control group(all P<0.05).The area under ROC curve(AUC)of miR-195 in patients with AMI was 0.94,and the sensitivity and specificity of miR-195 were 90.0%and 94.7%,respectively.The AUC,sensitivity and specificity of miR-195 combined with cTnI was 0.96,91.0%,and 93.0%,respectively.Bioinformatics analysis results showed that the intersection genes of miR-195 target genes and AMI disease-related genes were mostly enriched in the negative regulatory pathways of apoptosis. Conclusion MiR-195 has a good efficiency in early diagnosis of AMI which may be due to the involvement of miR-195 target gene in the regulation of apoptosis of myocardial cells.

关 键 词：急性心肌梗死 循环微小RNA 细胞凋亡 靶基因 

分 类 号：R542.2[医药卫生—心血管疾病]