牛场废水培养肥壮蹄形藻的工艺参数优化  

Process optimization of cultivation of Kirchneriella obesa in cattle farm wastewater

作　　者：王忠江[1,2] 王子越 王贵祥 郑宇 王丽丽 隋宇航[1] Wang Zhongjiang;Wang Ziyue;Wang Guixiang;Zheng Yu;Wang Lili;Sui Yuhang(College of Engineering,Northeast Agricultural University,Harbin 150030,China;Heilongjiang Key Laboratory of Technology and Equipment for the Utilization of Agricultural Renewable Resources,Harbin 150030,China)

机构地区：[1]东北农业大学工程学院,哈尔滨150030 [2]黑龙江省寒地农业可再生资源利用技术与装备重点实验室,哈尔滨150030

出　　处：《农业工程学报》2021年第24期257-265,共9页Transactions of the Chinese Society of Agricultural Engineering

基　　金：黑龙江省重点研发计划项目(GA21C024);国家高技术研究发展计划(2014AA022001)。

摘　　要：该研究选用肥壮蹄形藻(Kirchneriellaobesa)为试验藻种,以秸秆过滤后牛场废水与BG11培养基的混合物为微藻培养液,在前期单因素试验基础上研究废水添加比、光照强度、光照时间和通气量对微藻干重、氨氮去除率、总磷去除率和Chemical Oxygen Demand (COD)去除率的交互影响,通过Central Composite Design (CCD)中心组合试验得到了相应的影响模型。试验结果表明牛场废水培养肥壮蹄形藻的最优工艺条件为:废水添加比例26%、光照强度9 028 lx、光照时间21.5 h、通气量2.0 L/min。该研究采用优化工艺,在14 d的培养周期末期,肥壮蹄形藻的干重达到了1.141g/L,废水中氨氮去除率、总磷去除率和COD去除率分别达到了99.65%、99.15%、85.83%,响应值指标的预测值和实际值误差均在2%以内。各因素对目标值的交互作用关系为:废水添加比例和光照时间对肥壮蹄形藻干重的交互作用较强,废水添加比例和通气量对肥壮蹄形藻培养液中氨氮去除率、总磷去除率和COD去除率均具有较强的交互作用,而光照强度和光照时间仅对总磷去除率具有较强的交互作用。该研究为牛场废水培养微藻的工业化生产提供了理论参考。Water shortage and pollution are pushing to upgrade the conventional treatment of wastewater in the world.Microalgae-based Wastewater Treatment(WWT) has been widely expected to meet the new demand for the removal of nutrients and pathogens in recent years. However, a pretreatment process is normally required on the microalgae culture in wastewater from a cattle farm, such as settling, centrifugation, aeration, and dilution. It is a high demand for the potential microalgae culture substrates rich in nutrients, such as nitrogen and phosphorus, thereby reducing the cost of the pretreatment process for the better commercialization of the microalgae industry. Particularly, a large amount of breeding wastewater and manure have been rapidly produced in the livestock industry. In this study, the wastewater from a cattle farm was filtered by straw to cultivate the microalgae, in order to timely alleviate the loss of nutrients and environmental pollution. Kirchneriella obesa was selected as the experimental algae species. The mixture of straw-filtered wastewater and BG11 medium was also used as the microalgae culture medium. A systematic investigation was made on the effects of wastewater addition ratio, light intensity, photoperiod, and aeration on the dry weight of microalgae, as well as the ammonium(NH_(4)^(-)N), Total Phosphorus(TP), and Chemical Oxygen Demand(COD) removal rate. A Central Composite Design(CCD) with a single factor test was carried out to optimize the process parameters during cultivation. The results demonstrated promising potential of WWT in the nutrients removal from cattle farm wastewater. An optimal combination of process parameters for the cultivation was achieved as follows: 26 % of wastewater, light intensity of 9 028 lx, light time of 21.5 h, and the aeration intensity of 2.0 L/min. By the end of the 14-day culture period, the dry weight of Kirchneriella obesa reached 1.141 g/L under optimal conditions, where 99.65%, 99.15%, and 85.83% of NH_(4)^(-)N, TP, COD were removed, respectively. There w

关 键 词：废水 过滤 微藻培养 工艺优化 交互作用 

分 类 号：Q938[生物学—微生物学]