高效液相色谱法测定枇杷润喉糖中11个成分的含量  

Determination of 11 Main Components in Loquat Throat Candy by High Performance Liquid Chromatography

作　　者：尚姝 方方 刘园 SHANG Shu;FANG Fang;LIU Yuan(Jiangsu Institute for Food and Drug Control,Nanjing,Jiangsu 210019,China)

机构地区：[1]江苏省食品药品监督检验研究院,南京210019

出　　处：《药学与临床研究》2022年第1期31-34,共4页Pharmaceutical and Clinical Research

摘　　要：目的:建立高效液相色谱法测定清咽类保健食品枇杷润喉糖中山奈酚、槲皮素、高良姜素、橙皮苷、金丝桃苷、异槲皮苷、槲皮苷、芦丁、科罗索酸、齐墩果酸、熊果酸等11个成分的含量。方法:采用Kromasil C_(18)色谱柱,以0.1%磷酸溶液-乙腈为流动相,梯度洗脱,流速为1.0 mL·min^(-1),柱温:35℃,进样量:10μL。结果:11个成分在一定浓度范围内与峰面积线性关系均良好,r>0.9999,检测限在0.0087~0.3210μg·mL^(-1),定量限在0.0261~1.0699μg·mL^(-1),平均回收率为88.6%~115.9%。结论:该方法准确性好,灵敏度高,可用于枇杷润喉糖中11个成分的检测。Objective:To establish an HPLC method for the determination of 11 main components kaempferol,quercetin,galangic,hesperidin,hypericin,isoquercetin,quercetin,rutin,corosolic acid,oleanolic acid and ursolic acid in loquat throat candies.Methods:A Kromasil C_(18) column was used,with 0.1%phosphoric acid solution-acetonitrile as the mobile phase,gradient elution was performed at a flow rate of 1.0 mL·min^(-1).Column temperature was set at 35℃.Results:Linear relationships between each of the 11 component and their peak areas were good within tested concentration ranges,r>0.9999,respectively.The detection limits were 0.0087-0.3210μg·mL^(-1),the quantitation limits were 0.0261^(-1).0699μg·mL^(-1),and the average recovery ranges were 88.6%^(-1)15.9%.Conclusion:The method has good accuracy and sensitivity,it can be used for the determination of 11 main components in loquat throat candies.

关 键 词：高效液相色谱 清咽类保健食品 枇杷润喉糖 黄酮 三萜酸 

分 类 号：R927.2[医药卫生—药学]