防风通圣丸对特应性皮炎小鼠的保护作用  

Protective Effect of Fangfeng Tongsheng Pill(防风通圣丸)on Mice with Atopic Dermatitis

作　　者：陈桂芳 郝佳旭 高娟 杨卫平 孙赟 张丽娟 张祎 王睿睿 CHEN Guifang;HAO Jiaxu;GAJuan;YANG Weiping;SUN Yun;ZHANG Lijuan;ZHANG Yi;WANG Ruirui(Yunnan University of Chinese Medicine,Kunming 650500,Yunnan,China;Yunnan Province Engineering Laboratory for National Healthcare Theories and Products,Kunming 650500,Yunnan,China;Yunnan Provincial Pharmaceutical Company,Qujing 655000,Yunnan,China)

机构地区：[1]云南中医药大学,云南昆明650500 [2]云南省民族特色养生理论与健康产品工程实验室,云南昆明650500 [3]云南省医药公司,云南曲靖655000

出　　处：《中华中医药学刊》2022年第1期139-143,I0032-I0039,共13页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金(81760741,81660737,81760775);云南省科技厅中医联合重点项目(2017FF117[-003]);云南省科技厅联合专项(2017FF117[-025],2019FF002[-051]);云南省科技厅中医联合专项(2018FF001[-050],2019FF002[-010]);云南省教育厅科学研究基金(2020J0291)。

摘　　要：目的探讨防风通圣丸对特应性皮炎(atopic dermatitis,AD)小鼠的药效作用和初步作用机制。方法将BALB/c小鼠随机分为6组:空白对照组,模型对照组,阳性对照组(醋酸地塞米松2 mg/kg)及防风通圣丸低、中、高剂量(1、2、4 g/kg)组。除空白组外,其余5组小鼠均采用2,4-二硝基氯苯(DNCB)多次反复刺激皮肤建立AD模型,各组灌胃给予相应溶媒或受试药物,连续干预21 d。观察并评价AD模型小鼠背部皮肤皮损得分;剖取胸腺及脾脏计算脏器系数;苏木精-伊红(HE)染色法观察病理学变化,酶联免疫吸附法(Elisa法)测定小鼠外周血中总免疫球蛋白E(IgE)水平;甲苯胺蓝染色观察皮损组织中肥大细胞的浸润,免疫组化(IHC)法观察皮损组织中辅助T细胞2(Th2)型细胞因子的表达情况。结果在给予防风通圣丸后,AD模型小鼠湿疹样皮炎缓解,脾脏指数有下降的趋势。病理切片结果显示,小鼠皮肤表皮增厚减轻、炎性细胞数目减少及细胞间水肿减轻;血清IgE水平显著降低(P<0.01);真皮层中肥大细胞浸润数目减少。免疫组化结果表明,炎症细胞因子在皮肤中的表达显著降低。结论防风通圣丸对AD小鼠有保护作用,其机制与降低血清中总IgE水平,减少皮肤真皮层中肥大细胞的浸润、减轻Th2型细胞因子的表达有关。Objective To explore the pharmacodynamics and preliminary mechanism of Fangfeng Tongsheng Pill(防风通圣丸)in the treatment of atopic dermatitis(AD)mice.Methods The BALB/c mice were randomly divided into 6 groups:blank control group,model control group,positive control group(Dexamethasone Acetate:2 mg/kg)and Fangfeng Tongsheng Pill low,medium and high dose groups(1,2,4 g/kg).Except the blank control group,the mice of the other 5 groups were repeatedly stimulated the skin to establish an AD model by 2,4-dinitrochlorobenzene(DNCB).Each group was given the corresponding solvents or test drugs by gavage,and continued intervention for 21 days.The scores of skin tissue damage on the back of AD model mice were observed and evaluated.The thymus and spleen were cut to calculate the organ coefficient.Hematoxylin-eosin staining(HE)staining was used to observe pathological changes.Enzyme-linked immunosorbent assay(Elisa)method was used to measure the total Immunoglobulin E(IgE)level in peripheral blood of mice.Toluidine blue staining was used to observe the infiltration of mast cells.Immunohistochemistry(IHC)to observe the expression of helper T cell 2(Th2)cytokines in skin lesions.Results When AD model mice were treated with Fangfeng Tongsheng Pill,their eczema-like dermatitis was relieved.The spleen index had a downward trend.The pathological section results showed that the skin epidermis of the mice was reduced in thickening,the number of inflammatory cells and the intercellular edema were reduced.Serum IgE levels were significantly reduced(P<0.01).The number of mast cell infiltration in the dermis was decreased.The results of immunohistochemistry showed that the expression of inflammatory cytokines in the skin was significantly reduced.Conclusion Fangfeng Tongsheng Pill has a therapeutic effect on AD mice.The mechanism is related to reducing the total IgE level in the serum,the infiltration of mast cells in the skin dermis and the expression of Th2 cytokines.

关 键 词：特应性皮炎 防风通圣丸 肥大细胞 TH2型细胞因子 

分 类 号：R285.5[医药卫生—中药学]