HPLC法同时测定四逆散中8种成分  

Simultaneous determination of eight constituents in Sini Powder by HPLC

作　　者：贾可可 陈闪闪 许美娟[1] 杨欣怡 邹建东[1] 居文政[1] JIA Ke-ke;CHEN Shan-shan;XU Mei-juan;YANG Xin-yi;ZOU Jian-dong;JU Wen-zheng(Affiliated Hospital of Nanjing University of Chinese Medicine(Jiangsu Province Hospital of Chinese Medicine),Nanjing 210029,China;Nanjing University of Chinese Medicine,Nanjing 210029,China)

机构地区：[1]南京中医药大学附属医院(江苏省中医院),江苏南京210029 [2]南京中医药大学,江苏南京210029

出　　处：《中成药》2022年第2期372-375,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(81903823);中国博士后科学基金面上项目(2019M661903);江苏省博士后科研资助项目(2019K163);江苏省中医药领军人才项目(SLJ0208);江苏高校优势学科建设工程资助项目(苏政办发[2018]87);南京中医药大学中医学优势学科三期项目开放课题(ZYX03KF044)。

摘　　要：目的建立HPLC法同时测定四逆散(甘草、白芍、枳实等)中没食子酸、芍药内酯苷、芍药苷、橙皮苷、槲皮素、甘草酸、柠檬苦素、川陈皮素的含量。方法该药物20%甲醇提取液的分析采用Agilent ZORBAX SB-C_(18)色谱柱(4.6 mm×150 mm,5μm);流动相乙腈-水(含0.1%磷酸),梯度洗脱;体积流量1.0 mL/min;柱温35℃;检测波长210、240 nm。结果8种成分在各自范围内线性关系良好(R^(2)≥0.9990),平均加样回收率94.21%~100.12%,RSD 0.56%~2.41%。结论该方法简便、准确、稳定,重复性好,可用于四逆散的质量控制。AIM To establish an HPLC method for the simultaneous content determination of gallic acid,albiflorin,paeoniflorin,hesperidin,quercetin,glycyrrhizic acid,limonin and nobiletin in Sini Powder(Glycyrrhizae Radix et Rhizoma,Paeoniae Radix Alba,Aurantii Fructus Immaturus,etc.).METHODS The analysis of 20% methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent ZORBAX SB-C_(18) column(4.6 mm×150 mm,5 μm),with the mobile phase comprising of acetonitrile-water(containing 0.1% phosphoric acid) flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 210,240 nm.RESULTS Eight constituents showed good linear relationships within their own ranges(R^(2)≥0.999 0),whose average recoveries were 94.21%-100.12% with the RSDs of 0.56%-2.41%.CONCLUSION This simple,accurate,stable and reproducible method can be used for the quality control of Sini Powder.

关 键 词：四逆散 化学成分 HPLC 

分 类 号：R927.2[医药卫生—药学]