格氏束腰蟹(Somanniathelphusa grayi)的线粒体基因组序列测定和基因顺序进化研究  

The Mitochondrial Genome of Somanniathelphusa grayi and the Evolution of Gene Order

作　　者：杜诗雨 张康琴 潘达 孙红英[1] Du Shiyu;Zhang Kangqin;Pan Da;Sun Hongying(School of Life Sciences,Nanjing Normal University,Jiangsu Key Laboratory for Biodiversity and Biotechnology,Nanjing 210023,China)

机构地区：[1]南京师范大学生命科学学院,江苏省生物多样性与生物技术重点实验室,江苏南京210023

出　　处：《南京师大学报（自然科学版）》2022年第1期86-95,共10页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(31772427).

摘　　要：格氏束腰蟹(Somanniathelphusa grayi(Alcock,1909))(=Parathelphusa(Parathelphusa)chongi Wu,1935)是一种分布于中国云南和印度Moung Sal的束腰蟹属淡水蟹物种.本文使用高通量测序技术首次获得了该物种的线粒体基因组近全长序列,测定的线粒体基因组为一个未闭合的环状DNA,长度为17654 bp,包括37个基因(22个tRNA基因,13个蛋白质编码基因和2个rRNA基因),AT含量为72.8%(35.1%A,18.4%C,8.8%G,37.7%T).除trnS1-AGN缺少一个稳固的双氢尿嘧啶(DHU)臂外,其余tRNA基因的二级结构均为典型的三叶草结构.与短尾下目线粒体基因组的祖先排列顺序比较发现,该种线粒体基因组共涉及6个tRNA基因(trnR,trnN,trnF,trnP,trnQ和trnC)和1个蛋白质编码基因(nad5)的顺序变化,与此前报道的两种束腰蟹的基因排列顺序一致.这些基因的重排可以用置换和复制/随机丢失(TDRL)模型进行解释.研究发现,这种重排的线粒体基因组顺序还见于两种在我国江西和海南岛异域分布的束腰蟹种类.提示这种线粒体基因组基因排序是束腰蟹属重要的共近裔特征.本研究可以为格氏束腰蟹的分类厘订,以及束腰蟹属近缘物种的系统分类和拟地蟹科类群的分子进化研究提供新的分子证据.Somanniathelphusa grayi(Alcock,1909)(=Parathelphusa(Parathelphusa)chongi Wu,1935)was distributed in Yunnan,China and Moung Sal,India.The mitochondrial genome of S.grayi was firstly determined using high-throughput sequencing in this study.The sequenced mitochondrial genome of S.grayi was nearly-complete,17654 bp in length,containing 37 genes(22 tRNA genes,13 protein-coding genes and 2 rRNA genes).The AT nucleotide content was 72.8%(35.1%for A,18.4%for C,8.8%for G and 37.7%for T).The secondary structures of all tRNA genes were typical cloverleaf structures,except for trnS1-ARN,which lacked a dihydrouridine(DHU)arm.Compared with the brachyuran ground-pattern mitochondrial gene order,the mitochondrial gene order of S.grayi with the positions of six tRNAs(trnR,trnN,trnF,trnP,trnQ and trnC)and one PCGs(nad5)have been rearranged,which could be explained by transposition and TDRL models.The gene order was the same as the previously reported two Somanniathelphusa species.This gene order was shared by other two Somanniathelphusa species from Jiangxi and Hainan Island in China.It is suggested that this gene order pattern is one of the important common characteristics of the genus Somanniathelphusa,and it can provide the new molecular evidence for the systematic classification of Somanniathelphusa grayi,phylogenetic relationships within Somanniathelphusa and the molecular evolution of Gecarcinucidae.

关 键 词：格氏束腰蟹 淡水蟹 线粒体基因组 基因重排 

分 类 号：Q959[生物学—动物学]