中山市12株人源2型猪链球菌生物学特性研究及PFGE分型分析  

Biological characteristics and PFGE typing of 12 strains of humanStreptococcus suis type 2 in Zhongshan City

作　　者：饶文燕 郑悦康[1] 吴衍恒[1] RAO Wen-yan;ZHENG Yue-kang;WU Yan-heng(Zhongshan Center for Disease Control and Prevention,Zhongshang,Guangdong 528403,China)

机构地区：[1]广东省中山市疾病预防控制中心,广东中山528403

出　　处：《公共卫生与预防医学》2022年第2期16-20,共5页Journal of Public Health and Preventive Medicine

摘　　要：目的研究2016—2019年中山市人感染2型猪链球菌临床分离株的生物学特性及脉冲场凝胶电泳技术(pulsed field gel electrophoresis,PFGE)的分型,以期为临床防治猪链球病提供科学依据。方法收集2016-2019年人感染2型猪链球菌12株,采用全自动细菌鉴定仪对其进行鉴定,应用核酸蛋白分析仪检测其荚膜多糖(cps2J)、溶菌酶释放蛋白(mrp)、溶血素(sly)、谷氨酸脱氢酶(gdh)、胞外因子(ef)5个猪链球菌主要毒力基因的携带状况,应用微量肉汤稀释法测定猪链球菌对12种常用抗生素的敏感性,同时应用PFGE法对其进行同源性分析。结果12株2型猪链球菌分为4种毒力基因型,以mrp-/sly+/ef+/cps2J+/gdh+(6株)和mrp-/sly-/ef+/cps2J+/gdh+(4株)为主;药敏检测结果显示,12株2型猪链球菌对红霉素、四环素、克林霉素均耐药(耐药率为100.00%),且为多重耐药株,根据PFGE分型结果判定,按照100%的相似系数进行分型,12株猪链球菌分为7种PFGE型别,同一年份的猪链球菌PFGE带型具有较高的同源性。结论2016—2019年该市12株人感染2型猪链球菌临床分离株毒力基因型呈多样化,对多种抗生素耐药,同一年份的菌株多为同一克隆株,PFGE基因型与毒力基因型、耐药谱无关联性。Objective To study the biological characteristics of clinical isolates of human infected Streptococcus suis type 2 in Zhongshan City from 2016 to 2019 and classify the strains using pulse-field gel electrophoresis(PFGE),and to provide a scientific basis for clinical prevention and treatment of porcine streptococcus suis disease.Methods Twelve strains of Streptococcus suis type 2 were collected from 2016 to 2019 and identified by automatic bacterial identification instrument.The carrying status of five major virulence genes of Streptococcus suis was detected by nucleic acid and protein analyzer,including capsular polysaccharide(cps2J),lysozyme-releasing protein(mrp),hemolysin(sly),glutamate dehydrogenase(gdh),and extracellular factor(ef).The susceptibility of Streptococcus suis to 12 kinds of commonly used antibiotics was determined by the broth microdilution method,and the homology analysis was carried out by PFGE method.Results Twelve strains of Streptococcus suis type 2 were divided into four virulence genotypes,mainly mrp-/sly+/ef+/cps2J+/gdh+(6strains)and mrp-/sly-/ef+/cps2J+/gdh+(4strains).Drug susceptibility test results showed that 12 strains of Streptococcus suis type 2 were resistant to erythromycin,tetracycline and clindamycin,and they all were multi-resistant strains.According to the classification results of PFGE,the 12 strains were classified into 7 PFGE types based on 100%similarity coefficient.The PFGE band types of Streptococcus suis in the same year had high homology.Conclusion The virulence genotypes of 12 clinical isolates of human infected type 2 Streptococcus suis in Zhongshan from 2016 to 2019 are diverse,and the strains are resistant to multiple antibiotics.Most strains in the same year are the same clone strains.PFGE genotypes are not correlated with virulence genotypes and drug resistance spectrum.

关 键 词：2型猪链球菌 毒力基因 耐药性 

分 类 号：R181[医药卫生—流行病学]