线性和非线性定量在同位素稀释质谱法中应用的比较  

Comparison of a linear or nonlinear quantitative calibration for isotope dilution mass spectrometry method

作　　者：马妍慧[1] 皇甫昱婵[1] 周韵斓 沈立松[1] MA Yanhui;HUANG Fuyuchan;ZHOU Yunlan;SHEN Lisong(Department of Clinical Laboratory,Xinhua Hospital Affiliated to Medical College of Shanghai Jiao Tong University,Shanghai 200092,China)

机构地区：[1]上海交通大学医学院附属新华医院检验科,上海200092

出　　处：《国际检验医学杂志》2022年第5期523-527,533,共6页International Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目(31970860);上海医苑新星临床医技人才(临床检验专业)培养资助计划(沪医卫基〔2021〕99号)。

摘　　要：目的比较同位素稀释质谱法时,采用线性和非线性响应函数制作校准和(或)标准曲线对结果的影响。方法实验室建立的同位素稀释质谱法检测伊立替康及其3种主要代谢产物,使用C18反相柱梯度洗脱,以ABSciex API 3200检测。按《液相色谱-质谱临床应用建议》完成方法学验证。结果伊立替康(CPT-11)、SN-38G和APC在4.6~4.7 min出现洗脱峰,SN-38约在5.2 min出现洗脱峰。检测4种分析物的3种浓度质量控制(QC)样本,分别以线性函数、二次函数、三次函数和新的线性最小二乘法Padé[1,1]近似拟合分析,比较4种拟合方法计算得出的结果偏差。结论结果适合线性标准曲线时,优先应用线性;非线性校准曲线时,三参数有理函数一定程度上可以消除同位素稀释分析中不必要的误差源。Objective To determine the impact on the results of linear or nonlinear theoretical response functions calculated form isotope dilution mass spectrometry.Methods Irinotecan and its three main metabolites were detected by isotope dilution mass spectrometry established in the laboratory,which was eluted by C18 reverse phase column gradient and detected by ABSciex API 3200.The methodology validation was completed according to The recommendations for clinical application of liquid chromatography-mass spectrometry.Results CPT-11,SN-38G and APC showed elution peaks in 4.6-4.7 min,and the elution peak of SN-38 appeared in about 5.2 min.QC samples of three concentrations of four analytes were detected and approximate fitting analyzed by linear function,quadratic function,cubic function and new linear least squares Padé[1,1],respectively.The deviation of the results calculated by the four fitting methods was compared.Conclusion When the results are suitable for the linear standard curve,the linear standard curve is preferred.In the nonlinear calibration curve,the three parameter rational function can eliminate the unnecessary error source in isotope dilution analysis to a certain extent.

关 键 词：同位素稀释质谱法 校准曲线 线性函数 非线性函数 

分 类 号：O657.63[理学—分析化学]