基于转录组的不同火龙果品种抗性差异分析  

Resistance difference between different varieties of pitaya based on transcriptome data

作　　者：李健星 谭艳芳[1] 李冬兴[1,2] 王斌[1,2] 陈婷 黄甫昭[1,2] 陆树华[1,2] LI Jianxing;TAN Yanfang;LI Dongxing;WANG Bin;CHEN Ting;HUANG Fuzhao;LU Shuhua(Guangxi Institute of Botany,Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences,Guilin 541006,Guangxi,China;Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain,Guangxi Institute of Botany,Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences,Guilin 541006,Guangxi,China)

机构地区：[1]广西壮族自治区中国科学院广西植物研究所,广西桂林541006 [2]广西喀斯特植物保育与恢复生态学重点实验室,广西壮族自治区中国科学院广西植物研究所,广西桂林541006

出　　处：《广西植物》2022年第2期183-190,共8页Guihaia

基　　金：国家重点研发计划项目(2019YFC0507503,2019YFC0507504-03);广西创新驱动发展专项资金(桂科AA2016100403-01);广西自然科学基金(2019GXNSFBA245097);广西喀斯特植物保育与恢复生态学重点实验室基金(19-050-6)。

摘　　要：不同的品种抗性不同,为进一步探究不同火龙果品种之间的抗性差异,为后续火龙果抗性育种提供参考,该研究利用Illumina HiSeq 2000测序平台对‘普通白肉’(BR)和‘厄瓜多尔黄龙’(EY)两个品种进行转录组测序分析,并参考GO Ontology、KEGG等公共数据库对差异表达基因进行功能分类与富集分析。结果表明:(1)BR与EY共有14248个差异基因,其中5446个基因上调,8802个基因下调。(2)相关GO功能分析表明这些差异基因主要参与酶催化活性、细胞组分、代谢过程等,其中参与氧化还原酶活性的349个差异基因在BR中表达量上调。(3)KEGG通路分析显示,大部分差异基因富集在新陈代谢和生物合成等,其中参与角质、木栓质和蜡质生物合成的差异基因有12个,如CYP86和CER1等。参与氧化还原酶活性的差异基因在BR中较EY表达量上调,且显著富集,表明BR与EY在生长发育和细胞代谢过程差异显著。参与角质、木栓质和蜡质生物合成的差异基因在BR中表达量上调,此类基因在BR中具有较高的表达量且显著富集,表明BR可能具有较强的抗旱和抗病能力。Different varieties have different resistances.In order to further explore the resistance differences in different varieties of pitaya and to provide a reference for further study on breeding of pitaya resistance,we used Illumina HiSeq 2000 sequence platform to sequence the transcriptomes of‘Putongbairou’(BR)and‘Ecuador Yellow’(EY).Functional classification and enrichment analysis of differentially expressed genes(DEGs)were performed by reference to GO Ontology,KEGG and others databases.The results were as follows:(1)There were 14248 DEGs between BR and EY,of which 5446 genes were up-regulated and 8802 genes were down-regulated.(2)GO functional analysis showed that these DEGs were mainly involved in enzyme catalytic activity,cell components,metabolic processes,etc.Among them,there were 349 differential genes involved in oxidoreductase activity.(3)KEGG pathway analysis showed that most of the DEGs were enriched in metabolism,biosynthesis,etc.,and 12 key genes such as CYP86 and CER1 involving in cutin,suberine and wax biosynthesis.We found that the expressions of DEGs involved in oxidoreductase activity were higher in BR than those in EY,which significantly enriched,indicating that may be significant differences in growth and cell metabolism between BR and EY.DEGs involved in the cutin,suberine and wax biosynthesis were up-regulated in BR,and such genes had higher expressions in BR,and were significantly enriched,which suggest that BR may be superior drought and disease resistance than EY.

关 键 词：火龙果 转录组 品种 差异表达基因 

分 类 号：Q943[生物学—植物学]