依据CLSI EP07-A3文件评价血清指数对免疫散射比浊法检测前白蛋白的干扰  

Evaluation of the interference of serum index in the determination of prealbumin by immunoscattering turbidimetry according to CLSI EP07-A3 document

作　　者：韩光[1] 徐琼峰[2] 段淑敏 骆嘉欢 王云秀[1] 张秀娟[1] 万泽民[1] 柯培锋[1] 黄宪章[1] HAN Guang;XU Qiongfeng;DUAN Shumin;LUO Jiahuan;WANG Yunxiu;ZHANG Xiujuan;WAN Zemin;KE Peifeng;HUANG Xianzhang(Guangdong Provincial Hospital of Chinese Medicine,Guangzhou 510120,Guangdong,China;Aviation Health Center of China Southern Airlines Co.,Ltd.,Guangzhou 510400,Guangdong,China;Jinshazhou Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510168,Guangdong,China;Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,Guangdong,China)

机构地区：[1]广东省中医院,广东广州510120 [2]中国南方航空股份有限公司航空卫生中心,广东广州510400 [3]广州中医药大学金沙洲医院,广东广州510168 [4]中山大学孙逸仙纪念医院,广东广州510120

出　　处：《检验医学》2022年第2期108-111,共4页Laboratory Medicine

基　　金：广东省科技计划项目(20170215);广东省中医药局科研项目(20182056)。

摘　　要：目的探讨溶血、黄疸、脂浊对免疫散射比浊法检测前白蛋白(PA)的干扰效果。方法以新鲜血清样本作为基础样本,添加干扰物[血红蛋白(Hb)、胆红素及脂质]后,采用免疫散射比浊法检测PA水平。参考美国临床实验室标准化协会(CLSI)EP07-A3文件进行配对差异实验、剂量效应实验。结果10 g/L Hb(溶血指数为1000)、200 mg/L(340μmol/L)胆红素(黄疸指数为20)对PA的检测无干扰,脂浊指数为987的脂浊干扰物对PA检测有负干扰。脂质对PA的干扰程度与脂浊指数呈线性关系。对于PA低值样本,脂浊指数≤120时,对PA检测无显著干扰,脂浊指数与干扰率的线性方程为Y=-0.077X+0.979(r^(2)=0.999);对于PA高值样本,脂浊指数≤203时,对PA检测无显著干扰,脂浊指数与干扰率的线性方程为Y=-0.040X-0.168(r^(2)=0.992)。结论临床常见的溶血和黄疸样本不会干扰免疫散射比浊法PA检测结果,但脂浊指数>120会造成负干扰。Objective To investigate the interference effect of hemolysis,icterus and lipemia on the determination of prealbumin(PA)by immunoscattering turbidimetry.Methods A fresh serum sample was used as a basic sample,interfering substances[hemoglobin(Hb),bilirubin and lipid]were added,and the PA level was determined by immunoscattering turbidimetry.According to the EP07-A3 document of the Clinical and Laboratory Standards Institute(CLSI),the paired difference experiment and dose-effect experiment were performed.Results PA had no interference with 10 g/L Hb(hemolysis index of 1000)and 200 mg/L(340μmol/L)bilirubin(icterus index of 20).The lipid turbidity interfering substance with a lipid turbidity index of 987 had a negative interference on the determination of PA.The degree of interference had a linear relationship with the lipid turbidity index.For samples with low values of PA,when the lipid turbidity index≤120,there was no interference to PA determination.The linear equation between lipid turbidity index and interference rate was Y=-0.077X+0.979(r^(2)=0.999).For samples with high values of PA,when the lipid turbidity index≤203,there was no interference to PA determination.The linear equation between lipid turbidity index and interference rate was Y=-0.040X-0.168(r^(2)=0.992).Conclusions Generally clinical hemolytic and icteric samples will not interfere with the PA determination results by immunoscattering turbidimetry.The samples with lipid turbidity index>120 will cause negative interference to PA determination results.

关 键 词：前白蛋白 免疫散射比浊法 血清指数 干扰 

分 类 号：R446.1[医药卫生—诊断学]