基于特征算法的克罗诺杆菌OmpA蛋白的生物信息学分析  

Bioinformatic Analysis of Cronobacter Malonaticus OmpA Protein by Characteristic Algorithm

作　　者：李辉 涂胜倩 杨静[1] LI Hui;TU Sheng-qian;YANG Jing(Basic Experimental Teaching Center,Anhui Sanlian University,Hefei Anhui 230601)

机构地区：[1]安徽三联学院基础实验教学中心,安徽合肥230601

出　　处：《巢湖学院学报》2021年第6期98-105,共8页Journal of Chaohu University

基　　金：安徽三联学院质量工程项目(项目编号:20zlgc016);安徽省省级质量工程项目(项目编号:2020xfxm20);安徽三联学院校级科研重点项目(项目编号:KJZD2021008)。

摘　　要：目的对丙二酸盐克罗诺杆菌外膜蛋白OmpA蛋白的结构与功能进行预测与分析。方法从NCBI数据库获取丙二酸盐克罗诺杆菌外膜蛋白OmpA蛋白的氨基酸序列,利用ProtParam程序、ProtScal程序对OmpA蛋白的理化性质、亲疏水性进行分析,使用SignalP 5.0程序、TMHMM 2.0程序及NetPhos 3.1程序对OmpA蛋白的信号肽、跨膜结构域及磷酸化位点进行分析,运用改进的SOPMA算法、SWISS-MODEL程度对OmpA蛋白的二三级结构进行分析;同时,OmpA蛋白的B细胞/T细胞抗原表位、相互作用蛋白分别采用BepiPred程序/SYFPEITHI程序、STRING程序进行分析。结果丙二酸盐克罗诺杆菌LMG 23826由347个氨基酸组成,定义OmpA蛋白为稳定疏水性蛋白。该蛋白含有信号肽,不含有跨膜区,是一种外膜蛋白。结论OmpA蛋白为疏水性外膜蛋白,具有潜在的B、T细胞抗原表位,可为其抗原性研究和高效表位疫苗的研发提供理论参考。[Objective]To predict and analyze the structure and function of Cronobacter malonaticus protein OmpA.[Methods]The amino acid sequence of protein OmpA of Cronobacter malonaticus was obtained from the NCBI database.The physicochemical and hydrophilic properties of the protein OmpA were analysed by using ProtParam tool and ProtScal tool.SignalP 5.0 tool TMHMM 2.0 tool and NetPhos 3.1 were used to analyze the signal peptides,transmembrane regions,and phosphorylation sites of protein OmpA.The secondary structure of protein OmpA was predicted by using modified SOPMA algorithm.The tertiary structure of protein OmpA was predicted by using SWISS-MODEL tool.Meanwhile,B cell epitopes and T cell epitopes of protein OmpA were predicted by using BepiPred tool and SYFPEITHI tool.Interaction proteins of protein OmpA were analyzed by using STRING tool.[Results]The protein OmpA of Cronobacter malonaticus LMG 23826 contains 347 amino acids.The protein has the following characteristics:Its property is a stable hydrophobic protein.The protein has signal peptides but no transmembrane regions,indicating that it was located outside the membrane of bacteria.[Conclusion]The OmpA protein is hydrophobic puter membrane protein with potential B and T cell epitopes.These findings provide a theoretical reference for study of the antigenicity of Cronobacter malonaticus and for development of highly efficient epitope-based vaccines.

关 键 词：OmpA蛋白 结构 功能 特征算法程序 生物信息学 

分 类 号：R378.2[医药卫生—病原生物学]