不同检测方法对鲍曼不动杆菌、大肠埃希菌的鉴定结果比较及同源性分析  

Comparison of different detection methods for identification and homology analysis of Acinetobacter baumannii and Escherichia coli

作　　者：赵智龙 姚汶艺 宫海燕[1] 史松[3] ZHAO Zhilong;YAO Wenyi;GONG Haiyan;SHI Song(Department of Clinical Laboratory,Urumqi,Xinjiang 830011,China;Department of Nutrition,Urumqi,Xinjiang 830011,China;Department of Medical Administration,the Fifth Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang 830011,China)

机构地区：[1]新疆医科大学第五附属医院检验科,新疆乌鲁木齐830011 [2]新疆医科大学第五附属医院营养科,新疆乌鲁木齐830011 [3]新疆医科大学第五附属医院医务部,新疆乌鲁木齐830011

出　　处：《检验医学与临床》2022年第5期611-614,618,共5页Laboratory Medicine and Clinic

基　　金：新疆维吾尔自治区区域协同创新专项(科技援疆计划)项目(2019E0290);新疆维吾尔自治区研究生创新项目(XJ2021G232)。

摘　　要：目的比较基质辅助激光解析/电离飞行时间质谱(MALDI-TOF MS)、细菌生化鉴定两种方法对鲍曼不动杆菌、大肠埃希菌的鉴定能力差异,评价两种方法对鲍曼不动杆菌和大肠埃希菌鉴定的准确性。方法收集2019年3-4月临床分离的非重复鲍曼不动杆菌敏感菌株、大肠埃希菌敏感菌株各40株,经16S rRNA基因测序鉴定后,分别进行MALDI-TOF MS鉴定与细菌生化鉴定,并进行同源性分析。将16S rRNA基因测序鉴定方法作为金标准,对MALDI-TOF MS和细菌生化鉴定的准确性进行评价。结果40株鲍曼不动杆菌经MALDI-TOF MS鉴定,鲍曼不动杆菌19株、皮特不动杆菌12株、医院不动杆菌3株、抗辐射不动杆菌2株,4株未鉴定出;经细菌生化鉴定鲍曼不动杆菌40株。40株大肠埃希菌经MALDI-TOF MS、细菌生化鉴定均是大肠埃希菌。16S rRNA基因测序与MALDI-TOF MS鉴定鲍曼不动杆菌的一致性为90.00%,与细菌生化鉴定的一致性为47.50%;16S rRNA基因测序与MALDI-TOF MS、生化鉴定对大肠埃希菌的一致性均为100.00%。结论MALDI-TOF MS准确性较高,鉴定能力较强。细菌生化鉴定对鲍曼不动杆菌的鉴定分辨存在一定局限性,联合其他方法可提高鉴定率。Objective To compare the identification differences of Acinetobacter baumannii and Escherichia coli by Matrix assisted laser desorption/ionization mass spectrometry(MALDI-TOF MS)and bacterial biochemical identification,and to evaluate the accuracy of the identification of Acinetobacter baumannii and Escherichia coli.Methods A total of 40 non-repetitive Acinetobacter baumannii strains and 40 Escherichia coli strains were collected from March to April 2019.After identification by 16S rRNA gene sequencing,MALDI-TOF MS identification and bacterial biochemical identification were carried out,and homology analysis was conducted.16S rRNA gene sequencing was used as the gold standard,the accuracy of MALDI-TOF MS and bacterial biochemical identification was evaluated.Results The 40 strains of Acinetobacter baumannii were identified by MALDI-TOF MS,including 19 strains of Acinetobacter baumannii,12 strains of Acinetobacter Pitter,3 strains of Acinetobacter nosocomial and 2 strains of Acinetobacter radiophilus,4 strains were not identified.Those simples also were identified by bacterial biochemistry method,the results could only showed 40 strains of Acinetobacter baumannii.The 40 strains of Escherichia coli were identified as Escherichia coli by MALDI-TOF MS and bacterial biochemistry method.The consistency between 16S rRNA sequencing and MALDI-TOF MS for Acinetobacter baumannii was 90.00%,and the consistency between 16S rRNA gene sequencing and bacterial biochemical identification was 47.50%.The consistency of 16S rRNA gene sequencing,MALDI-TOF MS and bacterial biochemical identification for Escherichia coli was 100.00%.Conclusion MALDI-TOF MS has high accuracy and strong identification ability.However,there are some limitations in the identification of Acinetobacter baumannii by biochemical identification,the combined method can improve the identification rate.

关 键 词：基质辅助激光解析/电离飞行时间质谱 鲍曼不动杆菌 大肠埃希菌 同源性分析 

分 类 号：R446.5[医药卫生—诊断学]