昼夜温差对三角褐指藻和赤潮异弯藻生长和叶绿素荧光特性的影响  

EFFECTS OF DIURNAL TEMPERATURE DIFFERENCE ON GROWTH AND CHLOROPHYLL FLUORESCENCE OF PHAEODACTYLUM TRICORNUTUM AND HETEROSIGMA AKASHIWO

作　　者：李建安 王佳妤 梅熠辉 陈梅玲 周成旭[2] 李亚鹤[1] LI Jian-An;WANG Jia-Yu;MEI Yi-Hui;CHEN Mei-Ling;ZHOU Cheng-Xu;LI Ya-He(School of Marine Sciences,Ningbo University,Ningbo 315211,China;School of Food Sciences and Pharmacy Sciences,Ningbo University,Ningbo 315211,China)

机构地区：[1]宁波大学海洋学院,宁波315211 [2]宁波大学食品与药学学院,宁波315211

出　　处：《水生生物学报》2022年第2期194-202,共9页Acta Hydrobiologica Sinica

基　　金：国家重点研发计划“蓝色粮仓科技创新”专项(2018YFD0900702)资助。

摘　　要：为研究昼夜温差对三角褐指藻和赤潮异弯藻生长的影响,研究设置3个温度水平[培养温度为22℃、18℃和22—18℃(光-暗周期),分别记为22℃处理组、18℃处理组和22—18℃处理组]对2种藻进行分批和半连续培养。结果表明:(1)在3个温度处理下,分批培养时,和18℃相比,22—18℃降低了三角褐指藻在平台期的细胞密度,但不影响其延滞期的时长。半连续培养时,18℃处理下三角褐指藻的比生长速率最高;而对于赤潮异弯藻,分批培养时和22℃相比,22—18℃和18℃处理均延长了该藻延滞期的时长,但对其平台期细胞浓度的影响不显著,半连续培养时该藻比生长速率在22℃和18℃处理间无差异,而在22—18℃时显著降低;(2)在分批培养时,和22℃相比,低温18℃显著降低了二者的叶绿素a和类胡萝卜素含量;半连续培养时,温度不影响三角褐指藻的叶绿素a含量,但昼夜温差会显著提高赤潮异弯藻类胡萝卜素的含量(和22℃相比,增幅为24.5%);(3)无论是分批培养还是半连续培养,温度不影响2种藻光系统Ⅱ的最大光化学效率(F_(v)/F_(m)),但分批培养时高光[600μmol/(m^(2)·s)]条件下,2种藻光系统Ⅱ的有效光化学效率(Ф_(PSⅡ))和光化学猝灭(Qp)在18℃时略有增加;(4)2种藻均在22—18℃条件下具有较高的光合固碳速率。整体来说,三角褐指藻和赤潮异弯藻对昼夜温差的敏感性、响应和适应能力不同,这将在一定程度上影响环境变化条件下的浮游植物群落。研究从昼夜温差的角度出发,为深入探讨温度变化对浮游植物群落结构的影响提供了新思路。In order to investigate the effects of diurnal temperature difference on the growth of Phaeodactylum tricornutum and Heterosigma akashiwo,we cultured these two species batch and semi-continuously at three temperature levels(18℃:18℃for both light and dark period;22℃:22℃for both light and dark period;22—18℃:22℃for the light period and 18℃for the dark period).The results showed that:(1)Compared with 18℃,22—18℃reduced the cell density of P.tricornutum during batch culture at the plateau phase,but did not affect the latency time.In the semi-continuous culture,18℃had the highest specific growth rate(μ);for H.akashiwo,temperature had no significant effect on the cell concentration in the plateau stage,but 18℃and 22—18℃had prolonged latency time compared with 22℃,and 22—18℃significantly decreased the growth rate;(2)In batch cultivation,the chlorophyll a(Chl.a)and carotenoids(Car.)content of P.tricornutum and H.akashiwo decreased significantly in 18℃compared those in 22℃.Under semi-continuous culture,the temperature did not affect the Chl.a content,but 22—18℃increased the Car.content of H.akashiwo by 24.5%compared to 22℃treatment;(3)Temperature had no significant effects on the maximum effec-tive photochemical efficiency(F_(v)/F_(m))of photosystem II in P.tricornutum and H.akashiwo under both batch or semi-continuous culture;In batch cultivation,18℃showed higher effective photochemical efficiency(Ф_(PSⅡ))and photo-chemical fluorescence quenching(Qp)under 600μmol/(m^(2)·s)conditions than other 2 conditions;(4)Both P.tricornu-tum and H.akashiwo showed higher carbon fixation rates at 22-18℃.Thus,this study provides a new perspective to explore the influence of temperature changes on the structure of phytoplankton community.

关 键 词：三角褐指藻 赤潮异弯藻 昼夜温差 生长 叶绿素荧光参数 

分 类 号：Q948.11[生物学—植物学]