糖尿病肾脏病患者血清Sirt1与炎症及氧化应激反应、白蛋白尿短期进展的关系  

Relationship between serum Sirt1 and inflammation,oxidative stress,short-term progression of albuminuria in patients with diabetic kidney disease

作　　者：李靖[1] 叶卫丰[2] 徐安健[3] 张梅荣[1] 冯薇 LI Jing;YE Wei-feng;XU An-jian(Department of Health,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Department of Laboratory,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Scientific Research and Experiment Center,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China.)

机构地区：[1]首都医科大学附属北京友谊医院保健科,北京100050 [2]首都医科大学附属北京友谊医院检验科,北京100050 [3]首都医科大学附属北京友谊医院科研实验中心,北京100050 [4]首都医科大学附属北京友谊医院内分泌科,北京100050

出　　处：《临床和实验医学杂志》2022年第3期274-277,共4页Journal of Clinical and Experimental Medicine

基　　金：北京市自然科学基金(编号:7202034)。

摘　　要：目的研究糖尿病肾脏病(DKD)患者血清沉默信息调节因子1(Sirt1)与炎症及氧化应激反应、白蛋白尿短期进展的关系。方法回顾性选择2019年6月至2021年6月期间在首都医科大学附属北京友谊医院住院治疗的75例2型DKD患者作为DKD组,取同期就诊的60例单纯2型糖尿病患者作为DM组、体检的60名健康志愿者作为对照组。检测血清Sirt1及肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-6、8-羟基脱氧鸟苷(8-OHdG)、丙二醛、总抗氧化力(T-AOC)的含量,随访DKD患者白蛋白尿短期进展情况。结果DKD组、DM组患者血清中Sirt1的含量分别为(7.86±2.46)、(10.22±2.95)ng/mL,低于对照组[(14.17±3.21)ng/mL],且DKD组患者血清中Sirt1的含量低于DM组,差异均有统计学意义(P<0.05)。DKD组中24 h尿白蛋白>300 mg患者的血清Sirt1含量为(6.21±1.84)ng/mL,低于24 h尿白蛋白30~300 mg患者组[(8.51±2.85)ng/mL],差异有统计学意义(P<0.05)。DKD组中Sirt1含量≥中位数患者的血清TNF-α、IL-1β、IL-6、丙二醛、8-OHdG含量分别为(8.28±1.75)ng/mL、(4.58±0.83)ng/mL、(56.72±9.39)pg/mL、(6.58±1.15)μmol/L、(23.83±6.58)ng/mL,均低于Sirt1含量<中位数患者[(12.77±2.51)ng/mL、(7.03±1.76)ng/mL、(74.31±13.28)pg/mL、(8.93±1.52)μmol/L、(32.77±8.69)ng/mL],T-AOC含量为(11.39±2.55)U/mL,高于Sirt1含量<中位数患者[(8.37±1.74)U/mL],差异均有统计学意义(P<0.05)。Sirt1含量≥中位数患者的白蛋白尿短期进展累积发生率低于Sirt1含量<中位数患者,差异有统计学意义(P<0.05),且血清Sirt1含量对DKD患者白蛋白尿短期进展具有预测价值、最佳截断值为6.44 ng/mL。结论DKD患者血清Sirt1含量降低与炎症及氧化应激反应激活有关且能预测白蛋白尿短期进展。Objective To investigate the relationship between serum Sirt1 and inflammation,oxidative stress,short-term progression of albuminuria in patients with diabetic kidney disease(DKD).Methods From June 2019 to June 2021,75 type 2 diabetes patients with DKD were selected as DKD group,60 patients with simple type 2 diabetes were selected as DM group,and 60 healthy volunteers were selected as control group.Serum Sirt1,tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,IL-6,8-hydroxydeoxyguanosine(8-OHdG),malondialdehyde(MDA)and total anti-oxidant capacity(T-AOC)were detected.The short-term progress of albuminuria in patients with DKD was followed up.Results The levels of serum Sirt1 of patients in DKD group and DM group were(7.86±2.46)and(10.22±2.95)ng/mL,respectively,which were lower than those in control group[(14.17±3.21)ng/mL],and the level of serum Sirt1 of patients in DKD group were lower than those in DM group,and the differences were statistically significant(P<0.05).In DKD group,the level of serum Sirt1 of patients with 24 h urinary albumin>300 mg was(8.51±2.85)ng/mL,which was lower than of patients with 24 h urinary albumin 30-300 mg[(6.21±1.84)ng/mL],and the difference was statistically significant(P<0.05).The levels of serum TNF-α,IL-1β,IL-6,MDA,8-OHdG of patients with Sirt1 content≥median were(8.28±1.75)ng/mL,(4.58±0.83)ng/mL,(56.72±9.39)pg/mL,(6.58±1.15)μmol/L,(23.83±6.58)ng/mL,which were lower than that of patients with Sirt1 content<median[(12.77±2.51)ng/mL,(7.03±1.76)ng/mL,(74.31±13.28)pg/mL,(8.93±1.52)μmol/L,(32.77±8.69)ng/mL],the level of T-AOC was(11.39±2.55)U/mL,which was higher than that of patients with Sirt1 content<median[(8.37±1.74)U/mL],and the differences were statistically significant(P<0.05).The cumulative incidence of short-term progression of albuminuria of patients with Sirt1 content<median was higher than that of patients with Sirt1 content<median,and the difference was statistically significant(P<0.05).Serum Sirt1 content could predict the short-term progress o

关 键 词：糖尿病肾脏病 沉默信息调节因子1 炎症反应 氧化应激反应 白蛋白尿短期进展 

分 类 号：R587.2[医药卫生—内分泌] R692.9[医药卫生—内科学]