6种石斑鱼成鱼的性腺发育、脑垂体结构以及垂体中FSHβ和LHβ细胞的免疫识别  

Gonadal development, pituitary structures and immunohistochemical identification of FSHβ and LHβ cells in the pituitary in six species of the adult groupers

作　　者：徐文刚 刘立明[1] 王九龙 邹华锋 于文松 征矢野清 唐永政[1] XU Wengang;LIU Liming;WANG Jiulong;ZOU Huafeng;YU Wensong;SOYANO Kiyoshi;TANGYongzheng(School ofOcean,Yantai University,Yantai 264005,Shandong,China;Key Laboratory ofExploration and Utilization ofAquatic Genetic Resources,Ministry ofEducation,Shanghai Ocean University,Shanghai 201306,China;Yantai Institute ofMarine Economy,Yantai 264003,Shandong,China;Institute for East China Sea Research,Organization for Marine Science and Technology,Nagasaki University,Nagasaki 851-2213,Japan)

机构地区：[1]烟台大学海洋学院,山东烟台264005 [2]上海海洋大学水产种质资源发掘与利用教育部重点实验室,上海201306 [3]烟台市海洋经济研究院,山东烟台264003 [4]长崎大学环中国东海环境资源研究所,日本长崎851-2213

出　　处：《上海海洋大学学报》2022年第1期151-160,共10页Journal of Shanghai Ocean University

基　　金：日本学术振兴会(JSPS)研究项目(26660173);山东省农业重大应用技术创新项目(SD2019YY003);烟台大学博士科研启动基金(HX20B29)。

摘　　要：以日本海域捕获的宝石石斑鱼(Epinephelus areolatus)、黑边石斑鱼(E.fasciatus)、赤点石斑鱼(E.akaara)、尾纹九棘鲈(Cephalopholis urodeta)、蜂巢石斑鱼(E.merra)和纹波石斑鱼(E.ongus)成鱼为对象,比较其性腺发育和脑垂体结构以及垂体中FSHβ和LHβ免疫信号的分布。结果表明:宝石石斑鱼、黑边石斑鱼、赤点石斑鱼和尾纹九棘鲈性腺发育为未成熟阶段,蜂巢石斑鱼和纹波石斑鱼性腺发育为成熟阶段。6种石斑鱼的脑垂体位于间脑腹面,由神经垂体(NH)和腺垂体(AH)组成。腺垂体从左至右进一步细分为前外侧部(RPD)、中外侧部(PPD)和中间部(PI)。在宝石石斑鱼、赤点石斑鱼和纹波石斑鱼中NH结构被PPD隔开分为上下两部分,而在黑边石斑鱼、尾纹九棘鲈和蜂巢石斑鱼中NH为一个整体。6种石斑鱼脑垂体中FSHβ和LHβ细胞的免疫信号主要分布在PPD和PI区域,且FSHβ信号强度均较LHβ强,推断在石斑鱼性腺发育成熟前后,FSHβ较LHβ重要。为石斑鱼的资源保护及人工繁殖方面提供基础生物学资料和理论依据。The pituitary structures and the immune signals of FSHβ and LHβ of the six fish species(the areolate grouper Epinephelus areolatus, blacktip grouper E. fasciatus, red spotted grouper E. akaara, flagtail grouper Cephalopholis urodeta, honeycomb grouper E. merra and white-streaked grouper E. ongus) widely distributed along the coastline of Japan were investigated.The results showed: the gonadal development of areolate grouper, blacktip grouper, red spotted grouper and flagtail grouper was in the immature stage, however the honeycomb grouper and white-streaked grouper in the mature stage. The pituitary structures consisted of neurohypophysis(NH) and adenohypophysis(AH). The AH was further divided into the rostral pars distalis(RPD), the proximal pars distalis(PPD) and the pars intermedia(PI) from left to right. The immune signals of FSHβ and LHβ were detected in the PPD area or in the PPD and PI areas in six species, and the FSHβ signal intensity was stronger than the LHβ signal in six species. These results indicate that, FSHβ may play more important roles than LHβ in the gonadal development of six species when they were around mature stages. Based on the investigation of the six species of groupers along the coastline of Japan, the information of the interrelation between gonadal development and FSHβ and LHβ hormones function was obtained which may provide the basic biological data and theoretical basis for resource conservation and artificial propagation.

关 键 词：石斑鱼成鱼 性腺发育 脑垂体结构 FSHΒ LHβ 免疫组织化学 

分 类 号：S965.3[农业科学—水产养殖]