IL-17Rc基因敲除对小鼠椎体松质骨结构的影响  

Using Transgenic Mice to Explore the Effect of IL-17Rc Gene Knockout on Bone Metabolism

作　　者：虞凡 李博[1] 张国良 周松[1] YU Fan;LI Bo;ZHANG Guoliang

机构地区：[1]江西省南昌大学第一附属医院骨科,南昌330000

出　　处：《江西医药》2021年第12期2111-2115,共5页Jiangxi Medical Journal

基　　金：江西省自然科学基金-重点项目,编号20181ACB20024;江西省自然科学基金一般项目,编号20171BAB205031;国家自然科学基金地区科学基金项目,编号81560352;江西省卫计委中医药课题,编号2016A074。

摘　　要：目的利用转基因小鼠探讨IL-17Rc基因敲除是否导致椎体松质骨结构的变化。方法利用免疫组化技术micro-CT对比分析3月龄健康雌性野生型C57BL/6小鼠(对照组)与同龄同性别IL-17Rc基因敲除型C57BL/6小鼠(实验组)椎体松质骨的骨状态特征。结果野生型C57BL/6小鼠较mut-IL-17Rc基因型小鼠T13-L6相应椎体的松质骨内单位体积内骨密度更大(0.52±0.12vs.0.47±0.05,P=0.028)(g/cm^(3))、骨小梁连接数更多(8.97±1.46vs.5.59±3.15,P=0.017)(1/mm)、骨小梁厚度更厚(0.16±0.08vs.0.10±0.04,P=0.029)(μm)、骨小梁数量更多(5.01±0.33vs.3.16±0.37,P=0.038)(1/mm);此外,micro-CT的检测结果还提示:IL-17Rc基因敲除型C57BL/6小鼠骨质疏松主要表现在T13(P=0.039)、L1(P=0.035)、L3(P=0.018)、L5(P=0.021)、L6(P=0.036)椎体,L2(P=0.317)、L4(P=0.242)椎体的平均骨密度在两组小鼠间无明显统计学差异。结论IL-17/IL-17Rs信号轴广泛分布于动物的骨骼系统,且是调节骨代谢的重要信号通路之一;敲除IL-17Rc基因可导致模型动物骨质疏松的发生。Objective To investigate whether IL-17Rc gene knockout causes changes in the structure of pyramidal cancellous bone in transgenic mice.Methods The immunohistochemistry and micro-CT were used to analyze the condition of vertebral cancellous bone in 3-month healthy female wild-type C57BL/6 mice(control group)and IL-17Rc gene knockout C57BL/6 mice(experimental group).Results wild type C57BL/6 mice had higher bone density per unit volume(0.52±0.12 vs.0.47±0.05,P=0.028)(g/cm^(3)),more trabecular connections(8.97±1.46 vs.5.59±3.15,P=0.017)(1/mm),thicker trabecular thickness(0.16±0.08 vs.0.10±0.04,P=0.029)(μm)and the number of trabeculae was more(5.01±0.33 vs.3.16±0.37,P=0.038)(1/mm)than mut-IL-17Rc mice.In addition,the results of micro-CT also showed that the osteoporosis of IL-17Rc gene knockout C57BL/6 mice was mainly manifested in T13(P=0.039),L1(P=0.035),L3(P=0.018),L5(P=0.021)and L6(P=0.036),but the mean bone mineral density between L2(P=0.317)and L4(P=0.242)was no significant difference between the two groups.Conclusion IL-17/il-17rs signal axis is widely distributed in the animal skeletal system and is one of the important signal pathways regulating bone metabolism.Knockout of IL-17Rc gene can lead to the occurrence of osteoporosis in model animals.

关 键 词：IL-17Rc基因 IL-17/IL-17Rs信号轴 骨质疏松 小鼠 

分 类 号：R681.5[医药卫生—骨科学]