猪瘟病毒-猪圆环病毒2型疫苗联合免疫效果的评价  

Evaluation of the immune effect on co-inoculation of classical swine fever vaccine and porcine circovirus type 2 vaccines

作　　者：陈昌海[1] 连拯民 王相子[1] 徐小艳[1] 李向东 开妍[1] 邱冬 邵坤 CHEN Changhai;LIAN Zhengmin;WANG Xiangzi;XU Xiaoyan;LI Xiangdong;KAI Yan;QIU Dong;SHAO Kun(Jiangsu Province Animal Disease Prevention and Control Center,Nanjing 210036,China;College of Veterinary Medicine,Yangzhou University,Yangzhou 225000,China;Xuyi County Animal Disease Prevention and Control Center,Huai'an 211700,China)

机构地区：[1]江苏省动物疫病预防控制中心,江苏南京210036 [2]扬州大学兽医学院,江苏扬州225000 [3]盱眙县动物疫病预防控制中心,江苏淮安211700

出　　处：《畜牧与兽医》2022年第2期119-123,共5页Animal Husbandry & Veterinary Medicine

基　　金：江苏现代农业(生猪)产业技术体系疾病防控创新团队(JATS【2020】322)。

摘　　要：为建立一种既有效又简便的猪瘟和猪圆环病毒病疫苗联合免疫方法,通过细胞感染试验和动物免疫试验,评价了猪圆环病毒2型(PCV2)灭活疫苗和猪瘟病毒(CSFV)冻干活疫苗联合免疫的可行性。通过细胞感染试验证明了PCV2灭活疫苗稀释CSFV冻干活疫苗不影响CSFV疫苗的病毒活性;将PCV2灭活疫苗稀释的CSFV冻干活疫苗免疫试验猪群(联合免疫),以2种疫苗分边单独免疫的猪群为对照,在免疫后4、7、14、28、60和90 d采集血清检测2种疫苗对应的抗体,经统计学分析发现,2种疫苗的联合免疫不影响其抗体的产生,并且能够保持抗体在猪群个体间的稳定分布。In order to establish an more effective and convenient method for co-inoculation with the classical swine fever virus(CSFV) lyophilized live vaccine and the porcine circovirus type 2(PCV2) inactivated vaccine, the feasibility of co-injecting of CSFV lyophilized live vaccine and PCV2 killed vaccine was evaluated in vitro and in vivo. The results of the infected cells showed that the virus titers of the CSFV lyophilized live vaccine was not reduced by combining with the PCV2 inactivated vaccine. The CSFV lyophilized live vaccine and PCV2 inactivated vaccine were mixed and injected into pigs in one shot. Then, the CSFV and PCV2 specific antibodies were detected from the blood samples collected from the pigs at 4, 7, 14, 28, 60 and 90 d post vaccination. The results showed that, compared with individual vaccine immunization, CSFV and PCV2 specific antibodies were not affected by the co-vaccination in one shot, and the specific antibodies were evenly distributed in this manner of vaccination.

关 键 词：猪瘟病毒冻干活疫苗 猪圆环病毒2型灭活疫苗 联合免疫 抗体 

分 类 号：S852.6[农业科学—基础兽医学]