石墨炉法测定中药黄芪、人参中镉含量方法改进  

Improvement of the graphite furnace method for the determination of cadmium content in milkvetch root and ginseng

作　　者：孙冰雪[1] 于萍 李沅耕 崔贺铭 付雯雯[1] SUN Bingxue;YU Ping;LI Yuangeng;CUI Heming;FU Wenwen(School of Pharmacy,Jilin University,Changchun 130021,China)

机构地区：[1]吉林大学药学院,长春130021

出　　处：《长春中医药大学学报》2022年第3期292-294,共3页Journal of Changchun University of Chinese Medicine

基　　金：吉林大学实验技术立项项目(77)。

摘　　要：目的优化微波消解—石墨炉原子吸收法测定中药黄芪、人参中重金属镉的含量。方法将中药黄芪、人参粉碎过筛,采用微波消解法进行中药前处理。对基体改进剂、灰化温度及原子化温度等实验条件进行选择和优化,利用石墨炉原子吸收光谱法进行测定。结果选取氯化钯为基体改进剂,800 ℃为灰化温度,1 300℃为原子化温度进行测定。黄芪、人参中的镉含量在0~3 μg·L^(-1)浓度范围内,回归方程分别为Y=0.054 783X+0.007 5,Y=0.084 52X+0.036 5,相关系数(r)分别为0.999 1和0.998 7,该方法的线性关系良好,加标回收率为92.3%~109.7%,相对标准偏差(RSD)≤2.96%。结论改进后的方法灵敏度高、重现性好,可作为实验教学中药黄芪、人参中重金属镉含量的有效检测手段。Objective To optimize the microwave digestion-graphite furnace atomic absorption method for the determination of heavy metal cadmium in milkvetch root and ginseng.Methods Milkvetch root and ginseng were crushed and sieved,and then pretreated by the microwave digestion method.The experimental conditions such as matrix modifier,ashing temperature and atomization temperature were selected and optimized,and determined by the graphite furnace atomic absorption spectrometry.Results Palladium chloride was selected as the matrix modifier,the ashing temperature was 800 ℃,and the atomization temperature was 1 300 ℃ for measurement.The cadmium content in milkvetch root and ginseng was in the concentration range of 0 to 3 μg·L^(-1),and the regression equations were Y=0.054 783X+0.007 5,Y=0.084 52X+0.036 5,respectively.The correlation coefficients (r) were 0.999 1 and 0.998 7,respectively.The linear relationship of the method was good,with the recovery rate of standard addition 92.3% to 109.7%,and the relative standard deviation (RSD)≤2.96%.Conclusion The improved method has high sensitivity and good reproducibility,and can be used as an effective detection method for the cadmium content in milkvetch root and ginseng in experimental teaching.

关 键 词：微波消解 原子吸收 石墨炉 镉 

分 类 号：R284[医药卫生—中药学]