GATA结合蛋白3转录调控星形胶质细胞上调基因-1表达对子宫内膜癌细胞增殖､侵袭的影响  

Effects of GATA binding protein 3 on the proliferation and invasion of endometrial cancer cells through the transcriptional regulation of astrocyte elevated gene-1 expression

作　　者：蔡静[1] 姜黎黎 庞岚[2] CAI Jing;JIANG Lili;PANG Lan(Department of Gynecology,Handan Central Hospital,Handan 056000,Hebei,China;Department of Clinical Laboratory,Handan Maternal and Child Health Care Hospital,Handan 056001,Hebei,China)

机构地区：[1]邯郸市中心医院妇科,河北邯郸056000 [2]邯郸市妇幼保健院检验科,河北邯郸056001

出　　处：《中国性科学》2022年第2期89-94,共6页Chinese Journal of Human Sexuality

基　　金：河北省卫生健康委医学科学研究课题(20200441)。

摘　　要：目的研究GATA结合蛋白3(GATA3)转录调控星形胶质细胞上调基因-1(AEG-1)表达对子宫内膜癌细胞增殖、侵袭的影响。方法体外培养人子宫内膜癌原代培养细胞、人子宫内膜癌细胞系HEC-1-A、Ishikawa及HEC-1-B、人子宫内膜上皮细胞hEEC。将HEC-1-A细胞随机分为对照组、GATA3过表达质粒组、GATA3空载质粒组、GATA3 siRNA组、GATA3 siRNA阴性对照组,分组转染后,CCK-8实验评测各组细胞增殖情况;流式细胞实验评测各组细胞凋亡情况;细胞划痕、Transwell侵袭实验分别评测各组细胞迁移侵袭情况;qRT-PCR实验评测各组细胞GATA3与AEG-1的表达;免疫印记实验评测各组细胞凋亡蛋白caspase-9、Bax及EMT标志蛋白E-cadherin、Vimentin表达。同样方法分组转染人子宫内膜癌原代培养细胞后,检测各组细胞增殖、侵袭情况。结果与人子宫内膜上皮细胞比较,人子宫内膜癌原代培养细胞、人子宫内膜癌细胞系HEC-1-A、Ishikawa及HEC-1-B细胞中GATA3、AEG-1表达明显升高(P<0.05)。与对照组比较,GATA3过表达质粒组HEC-1-A细胞活力(包括人子宫内膜癌原代培养细胞)、迁移距离、侵袭细胞数(包括人子宫内膜癌原代培养细胞)、GATA3与AEG-1 mRNA表达水平、Vimentin表达升高(P<0.05),HEC-1-A细胞凋亡率、caspase-9、Bax及E-cadherin表达降低(P<0.05);GATA3 siRNA组呈相反趋势(P<0.05);GATA3空载质粒组、GATA3 siRNA阴性对照组各指标无明显差异(P>0.05)。结论下调GATA3表达,可降低AEG-1表达,抑制子宫内膜癌细胞增殖及侵袭迁移,促进其凋亡。Objective To study the effects of GATA binding protein 3(GATA3)on the proliferation and invasion of endometrial cancer(EC)cells through the transcriptional regulation of astrocyte elevated gene-1(AEG-1)expression.Methods Primary cultured human EC cells,human EC cell lines HEC-1-A,Ishikawa and HEC-1B,and human endometrial epithelial cells hEEC were cultured in vitro.HEC-1-A cells were randomly divided into control group,GATA3 overexpressed plasmid group,GATA3 no-load plasmid group,GATA3 siRNA group and GA-TA3 siRNA negative control group.After group transfection,cell proliferation of each group was evaluated by CCK-8 assay.Flow cytometry was used to evaluate the apoptosis of each group.Cell migration and invasion were evaluated by cell scratch and Transwell invasion assay.The expression of GATA3 and AEG-1 was evaluated by qRT-PCR.The expression of apoptosis protein caspase-9,Bax and EMT marker protein E-cadherin and Vimentin were evaluated by immunoimprinting assay.The proliferation and invasion of primary cultured human EC cells were detected after transfection by the same method.Results Compared with human endometrial epithelial cells,the expression of GATA3 and AEG-1 in human EC primary culture cells,human EC cell lines HEC-1-A,Ishikawa and HEC-1-B cells were significantly increased(P<0.05).Compared with the control group,HEC-1-A cell viability(including human EC primary culture cells),migration distance,number of invasive cells(including human EC primary culture cells),mRNA expression levels of GATA3 and AEG-1,and Vimentin expression were increased in GATA3 overexpressed plasmid group(P<0.05).The apoptosis rate of HEC-1-A cells and the expression of caspase-9,Bax and E-cadherin were decreased(P<0.05).GATA3 siRNA group showed an opposite trend(P<0.05).There were no significant differences between GATA3 no-load plasmid group and GATA3 siRNA negative control group(P>0.05).Conclusions Down-regulating the expression of GATA3 can reduce the expression of AEG-1,inhibit the proliferation,invasion and migration of EC c

关 键 词：GATA结合蛋白3 星形胶质细胞上调基因-1 子宫内膜癌 增殖 侵袭 

分 类 号：R711[医药卫生—妇产科学]