丹栀逍遥散对D-氨基半乳糖/脂多糖诱导的大鼠急性肝损伤保护作用机制研究  

Protective mechanism of Danzhi Xiaoyao powder on hepatic damage in rats with D-galactosamine/lipopolysaccharides-induced acute liver injury

作　　者：刘尚智[1] 张志强[1] 李昇锦 Liu Shangzhi;Zhang Zhiqiang;Li Shengjin(Department of Pharmacology,Sichuan College of Traditional Chinese Medicine,Mianyang 621000,Sichuan Province,China)

机构地区：[1]四川中医药高等专科学校药理学教研室,四川省绵阳市621000 [2]重庆医科大学附属第二医院

出　　处：《实用肝脏病杂志》2022年第2期179-182,共4页Journal of Practical Hepatology

基　　金：绵阳市重点研发计划科技支撑项目(编号:201802A208)。

摘　　要：目的探讨丹栀逍遥散对D-氨基半乳糖(D-GaIN)/脂多糖(LPS)诱导的大鼠急性肝损伤(ALI)的保护作用及其可能的作用机制。方法将110只SD大鼠随机分为对照组、模型组、小剂量、中剂量和大剂量丹栀逍遥散干预组,每组22只。采用D-GaIN)/LPS腹腔注射建立ALI模型,分别给予生理盐水或药物灌胃干预。采用ELISA法检测血清丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽-过氧化物酶(GSH-PX)、白介素-6(IL-6)、IL-1β、肿瘤坏死因子-α(TNF-α)水平,采用放射免疫分析法检测血清透明质酸酶(HA)、层黏连蛋白(LN)和Ⅲ型前胶原(Ⅲ-PC)水平。结果模型组血清AST、ALT、TBIL和MDA水平分别为(12081.3±587.7)U/L、(7658.7±742.2)U/L、(104.3±10.3)μmol/L和(8.9±1.1)mmol/mL,显著高于对照组【分别为(171.9±17.2)U/L、(60.5±6.2)U/L、(2.4±0.2)μmol/L和(2.5±0.3)mmol/mL,P<0.05】;模型组血清SOD和GSH-PX水平分别为(14.3±1.7)U/mL和(7.1±0.7)μmol/L,显著低于对照组【分别为(38.6±4.4)U/mL和(21.9±2.2)μmol/L】,而各剂量药物干预组血清SOD和GSH-PX水平显著高于模型组(P<0.05);模型组血清IL-6、IL-1β和TNF-α水平分别为(194.3±20.5)ng/mL、(24.7±2.5)ng/ml和(864.5±87.3)ng/mL,显著高于对照组【分别为(90.1±10.3)ng/mL、(5.2±1.0)ng/mL和(30.2±4.1)ng/mL】,而各剂量药物干预组血清细胞因子水平显著低于模型组(P<0.05);模型组血清HA、LN、Ⅲ-PC分别为(286.3±29.1)ng/ml、(297.4±30.5)ng/ml、(257.1±26.7)ng/ml,显著高于对照组【分别为(55.1±6.2)ng/mL、(75.1±7.6)ng/mL、(61.9±6.2)ng/mL】,而各剂量药物干预组血清肝纤维化指标水平显著低于模型组(P<0.05)。结论丹栀逍遥散对D-GaIN/LPS诱导的ALI大鼠肝功能损伤有保护作用,可能与其可明显抑制机体氧化应激反应和炎症反应有关。Objective The aim of this study was to explore the protective mechanism of Danzhi Xiaoyao powder on hepatic damage in rats with D-galactosamine(D-GaIN)/lipopolysaccharide(LPS)-induced acute liver injury(ALI).Methods 110 SD male rats were randomly divided into control,model,low-dose,moderate dose and large-dose of herbal medicine-intervened groups,with 22 rats in each.The ALI model was built-up by D-GaIN/LPS injection intraperitoneally,and the herbal medicine was given by lavage.Serum MDA,SOD,GSH-PX,IL-6,IL-1β,TNF-αlevels were detected by ELISA,and serum HA,LN andⅢ-PC levels were assayed by RIA.Results Serum AST,ALT,bilirubin and MDA levels in model group were(12081.3±587.7)U/L,(7658.7±742.2)U/L,(104.3±10.3)μmol/L and(8.9±1.1)mmol/mL,all significantly higher than[(171.9±17.2)U/L,(60.5±6.2)U/L,(2.4±0.2)μmol/L and(2.5±0.3)mmol/mL,respectively,P<0.05]in the control;serum SOD and GSH-PX levels were(14.3±1.7)U/mL and(7.1±0.7)μmol/L,both significantly lower than[(38.6±4.4)U/mL and(21.9±2.2)μmol/L,P<0.05]in the control,while serum SOD and GSH-PX levels in three herbal medicine-intervened groups significantly higher than those in the model(P<0.05);serum IL-6,IL-1βand TNF-αlevels in the model were(194.3±20.5)ng/mL,(24.7±2.5)ng/ml and(864.5±87.3)ng/mL,significantly higher than[(90.1±10.3)ng/mL,(5.2±1.0)n g/mL and(30.2±4.1)ng/mL in the control,while all significantly decreased compared to those in the model in three herbal medicine-intervened groups(P<0.05);serum HA,LN andⅢ-PC levels were(286.3±29.1)ng/ml,(297.4±30.5)ng/ml and(257.1±26.7)ng/ml,all significantly higher than[(55.1±6.2)ng/mL,(75.1±7.6)ng/mL and(61.9±6.2)ng/mL]in the control,while all significantly decreased compared to those in the model in three-herbal medicine-intervened groups(P<0.05).Conclusion Danzhi Xiaoyao powder could exert significant protective effect on liver functions in rats with D-GaIN/LPS-induced ALI,and the mechanism by which it works might be related to obvious inhibition of oxidative stress and inflammation

关 键 词：急性肝损伤 丹栀逍遥散 氨基半乳糖 脂多糖 大鼠 

分 类 号：R285.5[医药卫生—中药学]