利用染色体片段置换系群体定位和分析水稻粒重和粒型QTL  

Mapping and Analysis of QTLs for Rice Grain Weight and Grain Shape Using Chromosome Segment Substitution Line Population

作　　者：黄涛[1,2] 王燕宁 钟奇 程琴 杨朦朦 王鹏 吴光亮[1] 黄诗颖 李才敬 余剑峰 贺浩华 边建民[1,3] HUANG Tao;WANG Yanning;ZHONG Qi;CHENG Qin;YANG Mengmeng;WANG Peng;WU Guangliang;HUANG Shiying;LI Caijing;YU Jianfeng;HE Haohua;BIAN Jianmin(Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Nanchang 330045,China;College of Bioscience and Bioengineering,Jiangxi Agricultural University,Nanchang 330045,China;Jiangxi Super Rice Engineering Technology Research Center,Nanchang 330045,China)

机构地区：[1]作物生理生态与遗传育种教育部重点实验室,南昌330045 [2]江西农业大学生物科学与工程学院,南昌330045 [3]江西省水稻高水平工程研究中心,南昌330045

出　　处：《中国水稻科学》2022年第2期159-170,共12页Chinese Journal of Rice Science

基　　金：江西省杰出青年人才训练计划资助项目(20192BCB23010);江西省自然科学类重点项目(20192ACBL20017);江西省重大科技研发专项(20203ABC28W013);大学生创新创业项目(202010410105)。

摘　　要：【目的】挖掘水稻粒重和粒型相关性状QTL,对于解析水稻籽粒遗传机理具有重要作用。【方法】本研究以籼稻9311为受体、粳稻日本晴为供体构建的染色体片段置换系(Chromosome Segment Substitution Lines,CSSLs)群体为材料,在4个环境下对控制稻谷与糙米的粒重和粒型QTL进行了定位分析。【结果】共检测到77个控制水稻粒重和粒型的QTL,贡献率为4.62%~51.01%,其中19个QTL的增效等位基因来自日本晴,58个QTL的增效等位基因来自9311。这些QTL分布在水稻10条染色体的46个区域,其中16个区域为多效性位点。在两个及两个以上环境中重复检测到的QTL有14个,其中qGW5.1和qLW5能够在4个环境中稳定表达,且位于同一染色体区域;qBRL3.2和qGL4.1为新鉴定的影响水稻粒重和粒型的QTL。【结论】本研究结果为后续克隆这些QTL和解析水稻粒重和粒型遗传机理奠定了基础。【Objective】Mining quantitative trait loci(QTL)for grain weight and grain shape related traits in rice plays an important role in the genetic mechanism of rice grain.【Method】QTLs associated with grain weight and grain shape of grain and brown rice were analyzed using the population of chromosome segment substitution lines(CSSLs)with indica rice 9311 as recipient and japonica Nipponbare as donor in four different environments.【Result】A total of 77 QTLs for grain weight and grain shape of paddy and brown rice were detected,and the phenotypic variation explained by each QTL ranged from 4.62%to 51.01%.Among these QTLs,19 have additive alleles from Nipponbare and 58 have additive alleles from 9311.These QTLs distributed in 46 regions of 10 chromosomes in rice,and 16 regions are pleiotropic loci.There are 14 QTLs that can be repeatedly detected in two or more environments,qGW5.1 and qLW5 could be repeatedly detected in four environments and were located in the same chromosome region;qBRL3.2 and qGL4.1 were newly identified QTLs affecting grain weight and grain shape in rice.【Conclusion】The results laid a foundation for cloning these QTLs and analyzing the genetic mechanism of grain shape and grain weight in rice.

关 键 词：水稻 染色体片段置换系 粒重 粒型 QTL 

分 类 号：S511[农业科学—作物学]