不同引发剂对柴胡种子萌发的影响  

Effect of Different Initiator on Germination of Bupleurum chinense Seeds

作　　者：张鑫瑞 李政 孙哲 李澳旋 乔永刚[1] 郭淑红 宋芸[1] ZHANG Xinrui;LI Zheng;SUN Zhe;LI Aoxuan;QIAO Yonggang;GUO Shuhong;SONG Yun(College of Life Sciences,Shanxi Agricultural University,Taigu,Shanxi 030801;Institute of Cash Crops,Shanxi Agricultural University,Fenyang,Shanxi 032200,China)

机构地区：[1]山西农业大学生命科学学院,山西太谷030801 [2]山西农业大学经济作物研究所,山西汾阳032200

出　　处：《贵州农业科学》2022年第2期85-91,共7页Guizhou Agricultural Sciences

基　　金：国家重点研发计划项目“恒山黄芪、潞党参、北柴胡种质资源库构建与种质创制”(2019YFC1710801);吕梁市重点研发项目“北柴胡种质提纯及生态种植模式研究”(2020NYGG80)。

摘　　要：【目的】探明不同引发剂对柴胡种子萌发的影响,为其生产应用提供科学依据。【方法】选择赤霉素溶液、氯化钙溶液、PEG-6000溶液、硼酸溶液和水作为引发剂,研究不同浓度引发剂处理柴胡种子发芽率、发芽势、发芽指数和平均萌发时间的变化。【结果】4个引发剂各浓度溶液引发处理均可缩短柴胡种子的发芽进程,以100 mg/L赤霉素溶液、1.50%的氯化钙溶液、20%的PEG-6000溶液和250 mg/L硼酸溶液对柴胡种子的引发效果最好;水引发时间为8 h时,柴胡种子的各发芽指标均较CK提高;PEG-6000溶液和硼酸溶液引发柴胡种子的最早发芽时间最短,为10 d,较不引发处理(CK)提早4 d,赤霉素溶液、氯化钙溶液和水引发的最早发芽时间居中,均为11 d,较CK提早3 d。【结论】综合发芽率、发芽势、发芽指数和平均萌发时间,20%的PEG-6000溶液对柴胡种子的引发效果最好。【Objective】 The effect of different priming agents on germination of Bupleurum chinense seeds is discussed to provide the scientific basis for its application in B. chinense production.【Method】The effect of five priming agents(gibberellin, CaCl;, PEG-6000, boric acid and distilled water) with different concentrations on germination rate, germination vigor, germination index and average germination hours of B. chinense seeds.【Result】The gibberellin, CaCl;, PEG-6000 and boric acid solutions with different concentrations all can shorten the germination process of B. chinense seeds. The 100 mg/L gibberellin, 1.50% CaCl;, 20% PEG-6000 and 250 mg/L boric acid treatments all have the optimal priming effect of B. chinense seeds. The various germination indexes of B. chinense seeds soaked in distilled water for 8 h are higher than CK. The initial germination days of B. chinense seeds treated with PEG-6000 and boric acid solution are 10 d and 4 d earlier than CK. The initial germination days of B. chinense seeds treated with gibberellin and CaCl;solution and soaked in distilled water all are 11 d and 3 d earlier than CK.【Conclusion】The priming effect of B. chinense seeds treated with 20% PEG-6000 solution is the best among five priming agents according to the germination rate, germination vigor, germination index and average germination days of B. chinense seeds.

关 键 词：柴胡 种子 引发剂 发芽率 发芽势 发芽指数 

分 类 号：S567.79[农业科学—中草药栽培]