4种黄精属植物的基因组大小比较分析  

Comparative analysis on genome sizes of four Polygonatum species

作　　者：张苏炯 叶碧欢 陈友吾[2] 沈建军[2] 朱杰丽 李海波[2] ZHANG Sujiong;YE Bihuan;CHEN Youwu;SHEN Jianjun;ZHU Jieli;LI Haibo(Pan’an City Traditional Chinese Medicine Industry Development and Promotion Center,Pan’an,Zhejiang 322300,China;Zhejiang Academy of Forestry,Hangzhou,Zhejiang 310023,China)

机构地区：[1]磐安县中药产业发展促进中心,浙江磐安322300 [2]浙江省林业科学研究院,浙江杭州310023

出　　处：《森林与环境学报》2022年第2期193-198,共6页Journal of Forest and Environment

基　　金：浙江省中央财政林业科技推广项目(2021-TS16);浙江省衢州市科技攻关项目(2018-K45)。

摘　　要：为丰富黄精属(Polygonatum Mill.)植物的基因组大小数据,基于流式细胞术对源自不同产地的收录于药典的4种黄精属植物:滇黄精(P.kingianum)、黄精(P.sibiricum)、多花黄精(P.cyrtonema)和玉竹(P.odoratum)进行倍性检测和基因组大小估测。结果表明,毛竹(Phyllostachys pubescens)可作为分析黄精属植物基因组大小估测的合适内标;对于二倍体的4种黄精属植物,玉竹与滇黄精的基因组大小较为接近,玉竹为13 829~14 083 Mb,滇黄精为13 780 Mb;黄精的基因组略小,为12 792~13 516 Mb;多花黄精的基因组最小,为9 311 Mb。三倍体多花黄精的基因组大小为11 032~11 267 Mb,是二倍体植株的1.2倍。四倍体滇黄精的基因组大小为12 088~12 411 Mb,是二倍体植株的89%。本研究增补了黄精属植物的基因组大小数据,研究结果为黄精属植物后续的基因组学、物种分类以及种群进化研究提供基础参考数据。In order to enrich the data on genome sizes of Polygonatum species,flow cytometry was used for ploidy detection and estimation of genome sizes of four Polygonatum species recorded in the Chinese Pharmacopoeia,including P.kingianum,P.sibiricum,P.cyrtonema,and P.odoratum from different habitats.The results show that Phyllostachys pubescens can be used as a suitable reference standard for the estimation of genome sizes of Polygonatum species.Regarding the four diploid Polygonatum species,the present study found that the genome size of P.odoratum(13829-14083 Mb)is similar to that of P.kingianum(13780 Mb);the genome size of P.sibiricum(12792-13516 Mb)is slightly smaller than that of P.odoratum and P.kingianum;and the genome size of P.cyrtonema(9311 Mb)is the smallest.The genome size of triploid P.cyrtonema is 11032-11267 Mb,which is 1.2 times larger than that of diploid P.cyrtonema.The genome size of tetraploid P.kingianum is 12088-12411 Mb,which is 89%of the size of diploid P.kingianum.The results append the data on the genome sizes of Polygonatum species,which will provide a reference for a follow-up study on genomics,species classification,and population evolution.

关 键 词：黄精属 滇黄精 黄精 多花黄精 玉竹 基因组 

分 类 号：R282[医药卫生—中药学]