云南不明原因猝死地区隐花青鹅膏的毒性研究  

Toxicity study of amanita manginiana in Yunnan sudden unexplained death area

作　　者：杜进良 瞿鹏飞 李玉华 聂胜洁 段婷 习严梅 刘凯 马琳[3] 唐雪[3] 龙武 王蕊 瞿勇强 雷普平 王跃兵[3] Du Jinliang;Qu Pengfei;Li Yuhua;Nie Shengjie;Duan Ting;Xi Yanmei;Liu Kai;Ma Lin;Tang Xue;Long Wu;Wang Rui;Qu Yongqiang;Lei Puping;Wang Yuebing(School of Forensic Medicine,Kunming Medical University,Kunming 650500,China;School of Basic Medicine,Peking University Health Science Center,Beijing 100191,China;Yunnan Institute for Endemic Diseases Control and Prevention,Dali 671000,China)

机构地区：[1]昆明医科大学法医学院,公南昆明650500 [2]北京大学医学部基础医学院,北京100191 [3]云南省地方病防治所,云南大理671000

出　　处：《中国法医学杂志》2022年第1期54-59,共6页Chinese Journal of Forensic Medicine

基　　金：国家自然科学基金(81460285,81960573);昆医联合专项-重点项目(202101AY070001-008);昆明医科大学创新团队(CXTD201803)。

摘　　要：目的探究云南不明原因猝死案件事发当地隐花青鹅膏的毒性及毒理机制。方法采集事发地的隐花青鹅膏,经基因测序鉴定种属后制成干粉和熬煮物,SD大鼠灌胃染毒,提血清测定肝、肾功能以及心肌酶谱的生化指标。灌胃染毒7次后,取大鼠心肌、肝脏和肾脏组织进行HE染色和透射电镜观察。最后运用SPSS 21.0统计学软件分析数据。结果高剂量灌胃的两实验组大鼠在灌胃3次后出现活动减少、精神萎靡、食欲减退等症状。灌胃1次后各实验组的尿酸(Ua)、谷草转氨酶(AST)和谷丙转氨酶(ALT)均明显升高(P<0.05);两高剂量实验组的乳酸脱氢酶(LDH)、α-羟丁酸脱氢酶(α-HBDH)、肌酸激酶(CK)和肌酸激酶同工酶(CK-MB)显著升高(P<0.05)。HE染色发现,各实验组均出现心肌纤维断裂、肾小管上皮细胞水肿,熬煮物组肝细胞水肿明显,干粉组肝细胞脂肪变性、坏死,高剂量组的心肌、肝细胞和肾小管变化较明显。透射电镜发现,各实验组大鼠的肝细胞线粒体、粗面内质网损伤,心肌细胞线粒体损伤,干粉组还伴有溶酶体增多、脂滴现象,部分心肌原纤维断裂。结论YNSUD事发地的隐花青鹅膏对SD大鼠具有明显的肝毒性和心肌毒性,熬煮不能灭毒,食用具有一定的安全隐患,其心肌毒性可能是YNSUD发生的原因之一。Objective To investigate the toxicity of Amanita manginiana in Yunnan sudden unexplained death area through SD rats.Methods The Amanita lucidum from the site of the incident was collected.After the species were identified by gene sequencing,it was made to dry powder and boiled product.SD rats were gavaged with poison,and serum was collected to determine the biochemical indicators of liver and kidney function and myocardial enzyme spectrum.After intragastric administration for 7 times,the rat myocardium,liver and kidney tissues were taken for HE staining and transmission electron microscopy observation.Finally,SPSS 21.0 statistical software was used to analyze the data.Results The rats in the two experimental groups with high-dose intragastric administration showed symptoms of decreased activity,listlessness,and loss of appetite after three times of intragastric administrations.After intragastric administration,uric acid(Ua),Aspartate aminotransferase(AST)and alanine aminotransferase(ALT)were significantly increased in all experimental groups(P<0.05);lactate dehydrogenase(LDH),α-hydroxybutyrate dehydrogenase(α-HBDH),creatine kinase(CK)and creatine kinase isoenzyme(CK-MB)were significantly increased in the two high-dose experimental groups(P<0.05).HE staining showed that myocardial fiber rupture and renal tubular epithelial cell edema were found in all experimental groups.Hepatocyte edema was obvious in boiled food group,steatosis and necrosis of hepatocytes were obvious in dry powder group,and myocardium,hepatocytes and renal tubules obviously changed in in high dose group as well.Results from transmission electron microscopy indicated that the mitochondria of liver cells and rough endoplasmic reticulum in each experimental group were damaged,and the mitochondria of myocardial cells were damaged as well.The dry powder group was also accompanied by an increase in lysosomes and lipid droplets.Meanwhile,some myocardial fibrils were broken.Conclusion The Amanita manginiana in YNSUD area has obvious hepatotoxicity an

关 键 词：云南不明原因猝死 隐花青鹅膏 毒性作用 肝毒性 心肌毒性 

分 类 号：DF795.1[医药卫生—法医学]