乌鲁木齐市犬体表寄生蜱形态学及分子生物学鉴定  

Morphological and Molecular Biological Identification of Parasitic Tickson Dog Surface in Urumqi

作　　者：刘一凡 阿力木江·加帕尔 叶尔保勒 祖力亚·克力木江 韦丽婷 翟雪洁 巴音查汗·盖力克 王振宝 LIU Yi-fan;Alimujiang·Jiapaer;Yerbaole;Zuliya·Kelimujiang;WEI Li-ting;ZHAI Xue-jie;BaYinchahan·Gailike;WANG Zheng-bao(College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi,Xinjiang,830052,China;Yining Customs,Yining,Xinjiang,835000,China)

机构地区：[1]新疆农业大学动物医学学院,新疆乌鲁木齐830052 [2]伊宁海关,新疆伊宁835000

出　　处：《动物医学进展》2022年第2期85-89,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31660711)。

摘　　要：在乌鲁木齐市多家宠物医院、宠物店、犬舍、流浪犬基地、社区随机选取无明显临床症状的犬共1054只,采集其体表蜱虫889只,通过形态学鉴定及蜱虫的线粒体16S rDNA基因扩增序列进行分子生物学鉴定。鉴定得到假头基呈六角形的蜱虫,所采集的蜱虫初步鉴定为血红扇头蜱和图兰扇头蜱。发现血红扇头蜱和图兰扇头蜱的16S rDNA基因与已发表的国内河北株(JF979377)及新疆株(KU364373)同源性分别为97%和99%,与形态学鉴定结果一致。A total of 1054 dogs without obvious clinical symptoms were randomly selected from several pet hospitals,pet shops,kennels,stray dog bases and communities in Urumq,and 889 ticks were collected.The morphological identification and ticks mitochondrial 16S rDNA gene amplification sequence,molecular biological identification were conducted.Ticks with capitulum hexagon were identified,that the morphology of the recovered ticks were identified as Rhipicephalus sanguineus and R.turanicus.The 16S rDNA genes of R.sanguineus and R.turanicus were 97%homology to that of the published HeBei strain(JF979377)and 99%homology to that of the published Xinjiang strain(KU364373),which was consistent with the result of morphological identification.

关 键 词：犬 蜱虫 形态学鉴定 分子生物学鉴定 

分 类 号：S852.746[农业科学—基础兽医学]