复发性流产患者种植窗口期外周血与子宫内膜淋巴细胞亚群比例分析  

The analysis of the percentage of peripheral and endometrial lymphocyte subpopulation during implantation window period in patients with unexplained recurrent spontaneous abortions

作　　者：王耀楷 罗智华 姚吉龙[2] WANG Yao-kai;LUO Zhi-hua;YAO Ji-long(Guangzhou Medical University,Guangzhou,Guangdong 511436;Department of Obstetrics and Gynecology,Affiliated Shenzhen Maternity&Child Healthcare Hospital,Southern Medical University,Shenzhen,Guangdong 518000)

机构地区：[1]广州医科大学,广东广州511436 [2]南方医科大学附属深圳市妇幼保健院妇产科,广东深圳518000

出　　处：《赣南医学院学报》2022年第1期62-65,共4页JOURNAL OF GANNAN MEDICAL UNIVERSITY

摘　　要：目的:分析不明原因复发性流产(Unexplained recurrent spontaneous abortion,URSA)患者种植窗口期外周血及子宫内膜淋巴细胞亚群比例与URSA的关系。方法:流式细胞仪检测并分析20例URSA患者(观察组)和20例因男方因素行辅助生殖的妇女(对照组)种植窗口期外周血及子宫内膜中的CD3^(+)T淋巴细胞、CD3^(+)CD4^(+)T淋巴细胞、CD3^(+)CD8^(+)T淋巴细胞和CD3^(-)CD56^(+)CD16^(+)NK细胞比例。结果:⑴两组外周血CD3^(+)T淋巴细胞、CD3^(+)CD4^(+)T淋巴细胞、CD3^(+)CD8^(+)T淋巴细胞和CD3^(-)CD56^(+)CD16^(+)NK细胞比例差异无统计学意义(P>0.05);⑵观察组子宫内膜CD3^(+)T淋巴细胞比例高于对照组,CD3^(-)CD56^(+)CD16^(+)NK细胞比例低于对照组,差异有统计学意义(P<0.05),两组子宫内膜CD3^(+)CD4^(+)T淋巴细胞和CD3^(+)CD8^(+)T淋巴细胞比例差异无统计学意义(P>0.05);⑶两组外周血CD3^(+)T淋巴细胞比例和CD3^(-)CD56^(+)CD16^(+)NK细胞比例与子宫内膜相对应的淋巴细胞比例无相关性(P>0.05)。结论:子宫内膜局部种植窗口期淋巴细胞亚群比例失衡可能是URSA病因之一。Objective:To analyze the percentages of endometrial and peripheral lymphocyte subpopulation during"implantation window"period in patients with unexplained recurrent spontaneous(URSA).Methods:The 20 cases of patients with URSA in the observation group and 20 cases who performed assisted reproductive due to male factors in the control group were included in the study.Flow cytometry was used to detect the percentages of endometrial and peripheral T lymphocyte subpopulation(CD3^(+)T,CD3^(+)CD4^(+)T,CD3^(+)CD8^(+)T)and NK(CD3^(-)CD56^(+)CD16^(+)NK).Results:⑴RThe percentage of peripheral T lymphocyte subpopulation and NK cell had no significant difference(P>0.05)between observation group and control group;⑵the percentages of endometrial CD3^(+)T lymphocyte cell in observation group were significant higher than those of the control group,CD3^(-)CD56^(+)CD16^(+)NK was lower than that of the control group,with statistical significance(P<0.05),the endometrial CD3^(+)CD4^(+)T and CD3^(+)CD8^(+)T between the two groups had no statistical significance(P>0.05);⑶The proportion of peripheral lymphocytes CD3^(+)T and CD3^(-)CD56^(+)CD16^(+)NK corresponding to the endometrium had no correlation(P>0.05)between the two groups.Conclusion:The disorder of the percentages of endometrial lymphocyte subpopulation could be one of the causes of URSA.

关 键 词：不明原因复发性流产 子宫内膜 淋巴细胞 自然杀伤细胞 流式细胞仪 

分 类 号：R711.6[医药卫生—妇产科学]