甘蓝型油菜黄化转绿型突变系的光合特性  

Study on Photosynthetic Characteristics of Yellow Green-revertible Line of Brassica napus L.

作　　者：张耀文[1] 关周博[1] 李少钦[1] 侯君利[1] 董育红[1] 张文学[1] 田建华[1] ZHANG Yaowen;GUAN Zhoubo;LI Shaoqin;HOU Junli;DONG Yuhong;ZHANG Wenxue;TIAN Jianhua(Hybrid Rapeseed Research Center of Shaanxi Province, Shaanxi Rapeseed Branch of National Oil Crops GeneticImprovement Center,Yangling Shaanxi 712100, China)

机构地区：[1]陕西省杂交油菜研究中心,国家油料作物改良中心陕西油菜分中心,陕西杨凌712100

出　　处：《西北农业学报》2022年第3期257-269,共13页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省重点研发计划(2021NY-088)。

摘　　要：为解析甘蓝型油菜光合生理特性,以稳定遗传的黄化转绿型突变系和近等系为材料,对多种光合特性的变化规律进行了比较研究。结果表明:随着叶片发育,突变系与近等系的LPL、SPL叶绿素含量间的差异分别由70.34%、67.40%缩减为0.07%、0.17%;突变系叶绿素含量的增长速率比近等系高156.62%~727.03%、118.72%~7400.00%。突变系主要光合器官的4种光合色素含量均显著低于近等系。突变系叶片与角果的光饱和点、光补偿点、光量子效率、光呼吸速率、CO2饱和点、暗呼吸速率均显著高于近等系。突变系的LPL、SPL、SS的羧化效率均极显著低于近等系。突变系叶片的初始荧光、PSⅡ调节性能量耗散的量子产额、非光化学猝灭系数极显著高于近等系。突变系SS的PSⅡ调节性能量耗散的量子产额极显著高于近等系。突变系与近等系间LPL、SPL、SS的光合面积差异分别由(前期)25.85%、24.23%、14.15%逐步缩减为(后期)无差异。突变系的叶片数、角果数、株高、生物学产量仅在个别阶段显著低于近等系。在成熟期,突变系的分支部位、主花序长度显著低于近等系,而突变系的主花序角果数则显著高于近等系。甘蓝型油菜黄化转绿型突变系是一种具有较高研究利用价值的新型油菜叶色突变系。In order to analyze the photosynthetic physiological characteristics of Brassica napus L.,the changes of various photosynthetic characteristics were comparedand studied by using the stable and inheritable yellow-green revertible lines(Ygr)and near isogenic lines(Nil).The results showed that:with the development of leaves,the differences of chlorophyll content in the long-petiole leaf(LPL)and short-petiole leaf(SPL)ofthe Ygr and Nil reduced from 70.34%,67.40%to 0.07%,0.17%,respectively.The growth rates of chlorophyll content of the Ygr were 156.62%-727.03%and 118.72%-7400.00%higher than those of the Nil,respectively.The contents of chlorophyll a,chlorophyll b,chlorophyll(a+b)and carotene in the main photosynthetic organs of the Ygr were significantly lower than those of the Nil.The light saturation point,light compensation point,light quantum efficiency,light respiration rate,CO2 saturation point and dark respiration rate of leaf(LPL,SPL)and the light quantum efficiency,light respiration rate,light quantum efficiency of silique skin(SS)of the Ygr were significantly higher than those of the Nil.However,the carboxylation efficiency of the three types of photosynthetic organs in the Ygr was significantly lower than that in Nil.The initial fluorescence,quantum yield of PSⅡregulatory energy dissipation,non-photochemical quenching coefficient in leaf(LPL,SPL)of the Ygr were significantly higher than those of the Nil,while the potential activity of PSⅡ,photochemical quenching coefficient,the effective quantum yield of photochemical energy conversion and the apparent electron transfer rate of PSⅡin the leaves(LPL,SPL)of the Ygr were significantly lower than those of the Nil.The difference of the single leaf(LPL,SPL)and silique skin(SS)between the Ygr and Nil decreased by 25.85%,24.23%and 14.15%from the early stage to late stage of no difference,respectively.The number of leaves,the pod number,the plant height and the biological yield of the Ygr were significantly lower than those of the Nil only at some stages.At th

关 键 词：甘蓝型油菜 黄化转绿型突变系 光合特性 比较 利用 

分 类 号：S565.4[农业科学—作物学]