冷冻PN/A颗粒污泥快速活化过程中的污泥形态与菌群演化特征分析  

Characterization of Sludge Morphology and Bacterial Community Evolution in the Rapid Activation of Freeze-stored PN/A Granular Sludge

作　　者：黄子恒 张立 崔舒惠 张绮亮 文言 钱飞跃[1,2] HUANG Zi-heng;ZHANG Li;CUI Shu-hui;ZHANG Qi-liang;WEN Yan;QIAN Fei-yue(School of Environmental Science and Engineering,Suzhou University of Science and Technology,Suzhou 215009,China;National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology,Suzhou 215009,China)

机构地区：[1]苏州科技大学环境科学与工程学院,苏州215009 [2]城市生活污水资源化利用技术国家地方联合工程实验室,苏州215009

出　　处：《环境科学》2022年第2期920-927,共8页Environmental Science

基　　金：城市生活污水资源化利用技术国家地方联合工程实验室开放课题项目(2019KF02);江苏高校“青蓝工程”中青年优秀骨干教师培养对象项目;江苏省研究生创新计划项目(SJCX20_1101)。

摘　　要：接种颗粒污泥是快速启动高性能自养脱氮反应器的有效方法之一.为建立污泥活化与反应器效能的响应关系,在连续流反应器中接种冷冻储存的颗粒污泥,并采用高负荷和高水力选择压的调控策略,在34 d内成功启动了部分亚硝化/厌氧氨氧化(PN/A)一体化系统.反应器总氮去除率大于83%,总氮去除负荷稳定在1.67 kg·(m^(3)·d)^(-1).实验期间,使用Image pro-plus软件对颗粒的特征尺寸进行了分析.结果表明,颗粒内砖红色区域的投影面积与其总氮比去除速率呈现良好的线性正相关(R^(2)=0.988).这为快速评估PN/A污泥的活性提供了一种简易方法.由MiSeq高通量测序的结果可知,反应器运行在富集好氧氨氧化菌(Nitrosomonas)和淘洗异养菌(如Denitratisoma和Haliangium等)的同时,有效改善了颗粒密实度,为内部厌氧氨氧化菌(Candidatus_Kuenenia,丰度>30%)的活化创造了有利条件.Inoculating granular sludge is an alternative method for the quick start-up of a high-performance autotrophic nitrogen removal reactor.In order to establish the response relationship between sludge activation and reactor performance,the freeze-stored granular sludge was inoculated into a continuous-flow reactor,and a control strategy of the high loading rate and high hydraulic selective pressure was carried out in this study.As a result,a one-stage partial nitritation/ANAMMOX process was started up in 34 days,and the removal efficiency of total nitrogen was over 83%,with a removal loading rate of total nitrogen of 1.67 kg·(m^(3)·d)^(-1).During this period,the Image pro-plus software was employed to analyze the evolution of the characteristic dimensions of particles.A good linear positive correlation(R^(2)=0.988)between the projected area of the erythrine zone in the inner layer and the specific nitrogen removal rate of granules was found,which provide a simple method to estimate the activity of the PN/A granules.The results of MiSeq high-throughput sequencing showed that the enrichment of aerobic ammonia-oxidizing bacteria(Nitrosomonas)and the wash-out of heterotrophic bacteria(such as Denitratisoma and Haliangium,etc.)were achieved in the start-up of the reactor.Meanwhile,the improvement in granular compactness was in favor of activating anaerobic ammonia oxidizing bacteria(Candidatus_Kuenenia,abundance>30%)that colonized the inner layer of the granules.

关 键 词：厌氧氨氧化(ANAMMOX) 好氧颗粒污泥 颗粒形态 菌群结构 脱氮性能 

分 类 号：X703[环境科学与工程—环境工程]