基于Wnt3a/β-catenin信号通路探讨针刀对膝骨关节炎兔股直肌纤维化的影响  

Effects of acupotomy therapy on the fibroification of femoral rectus in rabbits with knee osteoarthritis based on Wnt3a/β-catenin signaling pathway

作　　者：刘晶 林巧璇 卢莉铭 郭泽兴 刘洪[1,3] 张良志[1,3] 赵红佳 修忠标[1,3,4] LIU Jing;LIN Qiao-xuan;LU Li-ming;GUO Ze-xing;LIU Hong;ZHANG Liang-zhi;ZHAO Hong-jia;XIU Zhong-biao(The People's Hospital Affiliated of Fujian University of Traditional Chinese Medicine,Fuzhou 350004,China;College of Traditional Chinese Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Key Laboratory of Bone Injury and Sports Rehabilitation of Traditional Chinese Medicine,Ministry of Education,Fuzhou 350122,China;Fujian Institute of Orthopaedics,Fuzhou 350004,China)

机构地区：[1]福建中医药大学附属人民医院,福州350004 [2]福建中医药大学中医学院,福州350122 [3]中医骨伤及运动康复教育部重点实验室,福州350122 [4]福建省骨伤研究所,福州350004

出　　处：《中华中医药杂志》2022年第1期136-140,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81873315,No.82004388);福建省科技厅卫生联合面上项目(No.2020J011038);国家中医临床研究基地专项科研课题(No.JDZX201906)。

摘　　要：目的:观察针刀干预对膝骨关节炎(KOA)兔股直肌Wnt3a/β-catenin信号通路调控作用及纤维化的影响,探讨针刀治疗KOA的可能作用机制。方法:24只健康雄性新西兰兔随机分为空白组、模型组、针刀组,每组8只。改良Videman法左后肢伸直位石膏固定制动6周复制KOA模型,Lequesne MG膝关节级别评分、X线和MRI确认造模成功后,采用经筋理论指导下在股四头肌鹤顶次、髌外上、髌内上经筋病灶点上行针刀松解治疗,每周1次,共治疗4次。采用Masson染色观察股直肌纤维化改变,Real-time PCR和Western Blot检测股直肌Wnt3a、β-catenin、TGF-β、CTGF、ColⅠmRNA和蛋白表达水平。结果:与空白组比较,模型组股直肌纤维化明显,胶原容积分数显著增加(P<0.05),Wnt3a、β-catenin、TGF-β、CTGF、ColⅠmRNA及蛋白表达水平显著升高(P<0.05);与模型组比较,针刀组股直肌纤维化减轻,胶原容积分数显著减小(P<0.05),Wnt3a、β-catenin、TGF-β、CTGF、ColⅠmRNA及蛋白表达水平显著降低(P<0.05)。结论:基于经筋理论针刀松解股四头肌经筋病灶点能通过调节Wnt3a/β-catenin信号通路,减轻KOA兔股直肌纤维化程度,这可能是针刀治疗KOA的作用机制之一。Objective:To observe the effect of acupotomy intervention on the regulation of Wnt3a/β-catenin signaling pathway and fibrosis in the rectus femora of rabbits with knee osteoarthritis(KOA),and to explore the possible mechanism of acupotomy in the treatment of KOA.Methods:Healthy male New Zealand rabbits were randomly divided into blank group,model group and acupotomy group,with 8 rabbits in each group.Modified Videman method was used to replicate the osteoarthritis model of the knee with left hind limb extended plaster immobility for 6 weeks.The blank group and model group were routinely fed and grabbed without intervention.In acupotomy group,the transfascial focal points of quadriceps femoris muscle were released by acupotomy under the guidance of jingjin theory,treatment points include Hedingci,Binwaishang,Binneishang.Once a week,a total of 4 times of treatment.Rectus femoris was taken and Masson staining was used to observe the morphological changes of rectus femoris.Real-time PCR and Western Blot was used to detect the mRNA and protein expression levels of Wnt3a,β-catenin,TGF-β,CTGF,Col I.Results:Compared with the blank group,the fibroification of the rectus femoris and the volume fraction of collagen were significantly increased in the model group(P<0.05).The mRNA and protein expression levels of Wnt3a,β-catenin,TGF-β,CTGF,Col I were significantly increased(P<0.05).Compared with the model group,the fibrillation of the rectus femoris was reduced and the volume fraction of collagen was significantly reduced in the acupotomy group(P<0.05).The mRNA and protein expression levels of Wnt3a,β-catenin,TGF-β,CTGF,Col I were significantly reduced(P<0.05).Conclusion:Based on jingjin theory,the release of quadriceps femoris by acupotomy can regulate the Wnt3a/β-catenin signaling pathway,to reduce the degree of femur rectus fibrosus in rabbits with KOA.This may be one of the mechanisms of acupotomy in treating KOA.

关 键 词：膝骨关节炎 针刀 经筋理论 骨骼肌纤维化 Wnt3a/β-catenin信号通路 机制 

分 类 号：R245[医药卫生—针灸推拿学]