四神丸对结肠炎小鼠结肠能量代谢水平调控机制  

Mechanism of Sishen Pill regulated colonic energy metabolism in colitis mice

作　　者：葛巍[1,2] 赵海梅[2,3] 李燕珍[4] 潘琦虹[4] 刘端勇[2,4] 王海燕[2] 程绍民[3] GE Wei;ZHAO Hai-mei;LI Yan-zhen;PAN Qi-hong;LIU Duan-yong;WANG Hai-yan;CHENG Shao-min(Affiliated Hospital of Jiangxi University of Chinese Medicine,Nanchang 330006,China;Formula-pattern Research Center,Jiangxi University of Chinese Medicine,Nanchang 330004,China;ColIege of Chinese Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,China;Science and Technology College,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学附属医院,南昌330006 [2]江西中医药大学方-证研究中心,南昌330004 [3]江西中医药大学中医学院,南昌330004 [4]江西中医药大学科技学院,南昌330004

出　　处：《中华中医药杂志》2022年第1期169-173,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81803988);江西省自然科学基金项目(No.20202BABL216062,No.20202ACBL206028,No.20192BAB215050,No.20181BAB205082);江西省教育厅科技计划项目(No.GJJ190646,No.GJJ196049)。

摘　　要：目的:探讨四神丸(SSP)对葡聚糖硫酸钠(DSS)诱导的结肠炎(UC)小鼠结肠组织能量代谢水平的影响。方法:将40只BALB/c雄性小鼠随机平均分为正常(Nor)组、模型(DSS)组、四神丸(SSP)组、美沙拉嗪(5-ASA)组。除Nor组外,其余均用DSS诱导UC小鼠模型,并于造模成功后第1天给予相应药物灌胃7 d。观察小鼠一般情况和结肠组织病理变化,分别采用考马斯亮蓝法检测总蛋白量、化学比色法检测ATP浓度、Na^(+)-K^(+)-ATPase和Ca^(2+)-Mg^(2+)-ATPase活力值变化,同时采用PCR array筛选ATP相关mRNA集中的基因/转录本并进行表达模式聚类分析和功能注释分析。结果:与DSS组比较,SSP组有效改善DSS诱导的UC小鼠一般情况和结肠组织病理损伤(P<0.01,P<0.05),同时伴见结肠组织ATP浓度、Na^(+)-K^(+)-ATPase和Ca^(2+)-Mg^(2+)-ATPase活力值提高(P<0.05),86个ATP相关的mRNA表达量出现不同程度的变化(P<0.01),其中,Atp5g1、Atp5e、Atp6v0c、Atp1a3 mRNA表达量与Nor组基本一致。结论:SSP可通过调节ATP浓度、ATP酶活力和ATP相关的mRNA表达量以改善线粒体能量代谢水平来治疗UC,其Atp5g1、Atp5e、Atp6v0c、Atp1a3能否成为靶分子尚需进一步验证。Objective:To investigate the effect of Sishen Pill(SSP)on energy metabolism in colonic tissue of mice with colitis induced by DSS.Methods:Forty BALB/c male mice were randomly divided into the normal group,model group,SSP group and 5-ASA group.The colitis model was established with TNBS/ethanol except the normal group,the SSP and 5-ASA were given on the first day after modeling for 7 days.The general conditions and the pathological changes of colon tissues were observed to evaluate the therapeutic effect of SSP,the total of ATP,Na^(+)-K^(+)-ATPase,Ca^(2+)-Mg^(2+)-ATPase in colonic tissue were tested by chemical colorimetry,and the GENES/transcripts of ATP-related mRNA concentration were screened by PCR array,expression pattern clustering analysis and functional annotation analysis were performed.Results:Compared with model group,the general conditions and the pathological injury of colon tissues in the SSP group were significantly improved(P<0.01,P<0.05),meanwhile,the concentrations of ATP and the activity of Na^(+)-K^(+)-ATPase,Ca^(2+)-Mg^(2+)-ATPase were increased(P<0.05),and the expression levels of 86 of ATP related mRNA were significantly changes(P<0.01),including Atp5g1,Atp5e,Atp6v0c,Atp1a3 mRNA were basically consistent with the normal group.Conclusion:SSP can improve mitochondrial energy metabolism level by regulating ATP concentration,ATPase activity and ATP-related mRNA expression to treat UC.However,whether Atp5g1,Atp5e,Atp6v0c and Atp1a3 can become target molecules still needs further verification.

关 键 词：四神丸 能量代谢 mRNA ATP 溃疡性结肠炎 机制 结肠组织 线粒体 

分 类 号：R285.5[医药卫生—中药学]