绞股蓝中皂苷类与黄酮类化合物的分离与鉴定  

Separation and identification of saponins and flavonoids in Gynostemma Pentaphyllum

作　　者：杨册 翟新房 段宇 齐彦爽 赵敏彤 朴香兰 YANG Ce;ZHAI Xin-fang;DUAN Yu;QI Yan-shuang;ZHAO Min-tong;PIAO Xiang-Ian(School of Pharmacy,Minzu University of China,Beijing 100081,China)

机构地区：[1]中央民族大学药学院,北京100081

出　　处：《中华中医药杂志》2022年第1期401-406,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81673692);国家自然科学基金中韩合作项目(No.81511140297);北京市大学生创新训练计划项目(No.BEIJ2020110030)。

摘　　要：目的:研究绞股蓝中皂苷类与黄酮类化合物的分离与鉴定。方法:采用硅胶、大孔树脂、中压柱色谱、半制备型HPLC及分析型HPLC等现代分离技术对绞股蓝的化学成分进行分离纯化,并根据其理化性质、UV、MS、NMR等方法进行结构鉴定。结果:从绞股蓝70%乙醇提取物中分离得到6个化合物,分别鉴定为gypenoside S5(1)、gypenoside XLIX(2)、gypenoside VN2(3)、ombuine(4)、yixingensin(5)、ombuoside(6)。结论:从绞股蓝中分离得到3个皂苷类和3个黄酮类化合物,其中化合物1为新化合物,命名为gypenoside S5。Objective:To study the separation and identification of saponins and flavonoids in Gynostemma Pentaphyllum.Methods:The chemical components of Gynostemma Pentaphyllum were separated and purified using modern separation technologies such as silica gel,macroporous resin,medium-pressure column chromatography,semi-preparative HPLC,and analytical HPLC.Their structures were identified by physical and chemical properties,UV,MS and NMR spectra.Results:Six compounds were isolated from the 70%ethanol extract of Gynostemma Pentaphyllum and were identified as gypenoside S5(1),gypenoside XLIX(2),gypenoside VN2(3),ombuine(4),yixingensin(5),and ombuoside(6),respectively.Conclusion:Three saponin compounds and three flavonoid are isolated from Gynostemma Pentaphyllum.From them,compound 1 is a new compound,named gypenoside S5.

关 键 词：绞股蓝 皂苷类化合物 黄酮类化合物 gypenoside S5 分离 纯化 结构鉴定 

分 类 号：R284.2[医药卫生—中药学]