低氧运动干预营养性肥胖模型大鼠骨骼肌能量代谢的变化  

Effects of hypoxic exercise on skeletal muscle energy metabolism of rats with alimentary obesity

作　　者：吴菊花[1] 杨亚南 翁锡全[3] 赵芳芳 徐国琴 林文弢[3,4] Wu Juhua;Yang Yanan;Weng Xiquan;Zhao Fangfang;Xu Guoqin;Lin Wentao(School of Physical Education,Guangxi University of Science and Technology,Liuzhou 545006,Guangxi Zhuang Autonomous Region,China;Guangdong Provincial Football Center,Guangzhou 510500,Guangdong Province,China;Department of Sports and Health,Guangzhou Sport University,Guangzhou 510500,Guangdong Province,China;Zhuhai College of Science and Technology,Zhuhai 519000,Guangdong Province,China)

机构地区：[1]广西科技大学体育学院,广西壮族自治区柳州市545006 [2]广东省足球运动中心,广东省广州市510500 [3]广州体育学院运动与健康系,广东省广州市510500 [4]珠海科技学院,广东省珠海市519000

出　　处：《中国组织工程研究》2022年第29期4598-4604,共7页Chinese Journal of Tissue Engineering Research

基　　金：广西科技大学运动与健康实验室基金项目(GKDTYSY2003),项目负责人:吴菊花;教育部人文社会科学研究青年基金西部和边疆项目(18XJCZH009),项目负责人:吴菊花;广西高校中青年教师基础能力提升项目(2018KY0314),项目负责人:吴菊花;广西科技大学博士基金(校科博17083),项目负责人:吴菊花。

摘　　要：背景:肥胖发生率逐渐攀升,易引起多种继发性疾病,对此,学者们提出众多减肥干预策略,其中运动干预或/和低氧暴露对肥胖防治具有较好的效果。目的:探讨低氧运动对肥胖大鼠骨骼肌过氧化物酶体增殖物激活受体α(peroxisome proliferators-activated receptorα,PPARα)/过氧化物酶体增殖物激活受体γ共激活分子1α(peroxisome proliferator-activated receptor-γcoactivator-1α,PGC-1α)/脂素1表达的影响。方法:构建营养性肥胖大鼠模型,建模后随机分为常氧安静组和运动组、体积分数16.3%低氧安静组和运动组、13.3%低氧安静组和运动组,继续高脂饲养,运动组进行8周耐力运动,20 m/min,40 min/d,每周5 d。末次运动24 h后麻醉处死大鼠并采样,Western blot及免疫组化测定大鼠骨骼肌PGC-1α、PPARα以及脂素1蛋白表达水平。结果与结论:①7周高脂膳食诱导营养性肥胖大鼠模型建立;②与常氧安静组相比,常氧运动组、16.3%低氧运动组及13.3%低氧运动组大鼠骨骼肌PGC-1α蛋白表达水平均显著升高(P<0.05-0.01);与16.3%低氧安静组相比,16.3%低氧运动组大鼠骨骼肌PGC-1α蛋白表达水平显著升高(P<0.05);与13.3%低氧运动组相比,常氧运动组、16.3%低氧安静组、13.3%低氧安静组大鼠骨骼肌PGC-1α蛋白表达水平均显著降低(P<0.05-0.01);③与常氧安静组相比,常氧运动组、13.3%低氧安静组和运动组大鼠骨骼肌PPARα蛋白表达水平显著升高(P<0.05-0.01);与13.3%低氧运动组相比,16.3%低氧安静组和运动组大鼠骨骼肌PPARα蛋白表达水平显著降低(P<0.05);④与常氧安静组相比,其他5组大鼠骨骼肌脂素1蛋白表达水平均有所降低,其中13.3%低氧运动组降低最为显著(P<0.05);⑤提示低氧运动可引起肥胖大鼠骨骼肌PGC-1α、PPARα蛋白表达增加,下调脂素1蛋白表达,促进骨骼肌脂肪酸氧化代谢,从而有利于改善肥胖大鼠骨骼肌的能量代谢。BACKGROUND:The incidence of obesity is gradually increasing,which is prone to cause a variety of secondary diseases.Therefore,numerous weight-loss intervention strategies have been proposed,among which exercise intervention or/and hypoxic exposure have better effects on the prevention and treatment of obesity.OBJECTIVE:To explo re the effect of hypoxic exercise on the protein expression levels of peroxisome proliferato rs-activated receptor α(PPARα)/peroxisome proliferato r-activated receptor-y coactivator-1α(PGC-1)/Lipin1 in the skeletal muscle of rats with alimentary obesity.METHODS:Animal models of alimentary obesity were established in rats,and the rat models were then randomized into normoxic quiet group and exe rcise gro up,16.3% hypoxic quiet group and exercise group,and 13.3% hypoxic quiet group and exercise group.The rats were fed with high fat,and all exe rcise gro ups received an endurance exercise,20 m/min,40 min/d,5 days per week for 8 wee ks.The rats were killed under anesthesia and sampled at 24 hours after the last exercise.The protein expression levels of PGC-1α,P PARα,and Lipin1 in the skeletal muscle of rats were then determined by western blot and immunohistochemistry.RESULTS AND CONCLUSION:The rat model of alimentary obesity was induced by a 7-week high-fat diet.Compared with the normoxic quiet group,the expression of PGC-1α protein in the skeletal muscle of rats was significantly increased in the normoxic exercise group,16.3% hypoxic exercise group,and13.3% hypoxic exercise group(P <0.05-0.01).Compared with the 16.3% hypoxic quiet group,the expression of PGC-1α protein in the skeletal muscle of rats was significantly increased in the 16.3% hypoxic exercise group(P<0.05).Compared with the 13.3% hypoxic exercise group,the expression of PGC-1α protein in the skeletal muscle of rats was significantly reduced in the normoxic exercise group,16.3% hypoxic quiet group,and 13.3% hypoxic quiet group(P <0.05-0.01).Compared with the normoxic quiet group,the expression of PPARα protein in the sk

关 键 词：低氧运动 肥胖 PGC-1Α PPARΑ 脂素1 骨骼肌 大鼠 

分 类 号：R459.9[医药卫生—治疗学] R87[医药卫生—临床医学]