2型糖尿病伴牙周炎模型大鼠胰腺自噬及胰岛素相关基因蛋白的表达  

Pancreatic autophagy and protein expression of insulin-related genes in type 2 diabetic rats with periodontitis

作　　者：都沙沙 蔡智国 杨琨 刘琪 Du Shasha;Cai Zhiguo;Yang Kun;Liu Qi(Department of Periodontology,Hospital of Stomatology,Zunyi Medical University,Zunyi 563003,Guizhou Province,China)

机构地区：[1]遵义医科大学附属口腔医院牙周科,贵州省遵义市563003

出　　处：《中国组织工程研究》2022年第29期4605-4610,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(81860196),课题名称:2型糖尿病伴牙周炎牙周膜干细胞凋亡转归的线粒体损伤、自噬失调机制研究,项目负责人:刘琪

摘　　要：背景:糖尿病易引发全身多种器官发生病变,牙周炎是其并发症之一,牙周炎炎症程度越重,越不利于血糖控制。目的:通过建立动物模型探索牙周炎对2型糖尿病大鼠胰腺组织自噬的影响。方法:40只雄性6周龄SD大鼠随机分为对照组和实验组,又将实验组分为糖尿病组、牙周炎组和糖尿病伴牙周炎组,每组10只。高脂高糖喂养+腹腔注射链脲佐菌素构建糖尿病模型,丝线结扎法构建牙周炎模型。ELISA法检测血清中炎症因子表达水平;实时荧光定量PCR检测胰腺组织中自噬及胰岛素分泌相关基因的表达;Western Blot法检测胰腺组织中自噬相关蛋白的表达。结果与结论:①ELISA结果显示,糖尿病组、牙周炎组及糖尿病伴牙周炎组的肿瘤坏死因子α、白细胞介素1β、白细胞介素6表达水平较对照组依次增加(P<0.05);②实时荧光定量PCR结果显示,与对照组相比,糖尿病组、糖尿病伴牙周炎组的葡萄糖激酶、葡萄糖转运蛋白2 mRNA表达水平均下降,糖尿病伴牙周炎组的表达下降最多(P<0.05);同时糖尿病组、糖尿病伴牙周炎组的解偶联蛋白2、Beclin1、LC3ⅡmRNA表达均增加,糖尿病伴牙周炎组的表达增加最多(P<0.05);但牙周炎组与对照组各指标相比差异无显著性意义(P>0.05);③Western Blot结果显示,相较于对照组,糖尿病组、糖尿病伴牙周炎组的Beclin1、LC3Ⅱ/LC3Ⅰ表达均增加,糖尿病伴牙周炎组表达增加最多(P<0.05);但牙周炎组与对照组相比差异无显著性意义(P>0.05);④推测2型糖尿病大鼠患牙周炎后可能诱导其胰岛细胞过度自噬,进而影响胰岛细胞分泌胰岛素的功能,最终加剧糖尿病病情。BACKGROUND:Diabetes is prone to cause multiple organ diseases in the body.Periodontitis is one of the complications.The more severe the inflammation of periodontitis is,the less favorable it is for glycemic control.OBJECTIVE:To investigate the effect of periodontitis on autophagy of pancreatic tissue in a rat model of type 2 diabetes mellitus.METHODS:Forty 6-week-old male Sprague-Dawley rats were randomly divided into a control group and an experimental group,and rats in the experimental group were subdivided into three groups(n=10 per group):a diabetes group,a periodontitis group,and a diabetes+periodontitis group.High fat and high sugar feeding+intraperitoneal injection of streptozotocin was used to establish a diabetic model,and silk thread ligation was used to establish a periodontitis model.Enzyme-linked immune sorbent assay was used to detect the expression of inflammatory factors in rat serum.Real-time fluorescent quantitative PCR was used to detect the expression of autophagy and insulin secretion-related genes in rat pancreatic tissue.Western blot was used to detect the protein expression of autophagy-related in rat pancreatic tissue.RESULTS AND CONCLUSION:The results of enzyme-linked immunosorbent assay showed that the expression of tumor necrosis factorα,interleukin 1β,and interleukin 6 was increased sequentially in the diabetes group,the periodontitis group,and the diabetes+periodontitis group compared with that in the control group(P<0.05).The results of real-time fluorescent quantitative PCR showed that,compared with the control group,the mRNA expression of glucokinase and recombinant glucose transporter 2 was decreased in the diabetes group and the diabetes+periodontitis group,especially in the diabetes+periodontitis group(P<0.05).At the same time,the mRNA expression of uncoupling protein 2,Beclin1,and LC3II was increased in the diabetes group and the diabetes+periodontitis group,and increased most in the diabetes+periodontitis group(P<0.05).However,there was no significant difference between the

关 键 词：糖尿病 牙周炎 自噬 胰岛素分泌 炎性因子 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]