促生菌对基质栽培番茄根系细菌群落的影响  

Impacts of growth-promoting bacteria on root bacterial community of tomato in substrate culture

作　　者：李凤 周方园 张广志 周红姿 吴晓青 吴金娟 张新建 LI Feng;ZHOU Fangyuan;ZHANG Guangzhi;ZHOU Hongzi;WU Xiaoqing;WU Jinjuan;ZHANG Xinjian(Shandong Provincial Key Laboratory of Applied Microbiology,Ecology Institute,Qilu University of Technology(Shandong Academy of Sciences),Ji’nan 250103,Shandong,China;Shandong Shangdao Biotechnology Limited Company,Ji’nan 250000,Shandong,China)

机构地区：[1]齐鲁工业大学(山东省科学院)生态研究所山东省应用微生物重点实验室,山东济南250103 [2]山东商道生物科技股份有限公司,山东济南250000

出　　处：《微生物学通报》2022年第2期583-597,共15页Microbiology China

基　　金：山东省重点研发计划(2019JZZY020610,2019GSF107086)。

摘　　要：[背景]植物促生菌剂产品在农业生产上的应用越来越广泛,但人们对促生菌在基质栽培条件下对作物根系微生物群落影响的了解有限。[目的]明确在基质栽培条件下促生菌假单胞菌(Pseudomonas sp.)JP2-3和枯草芽孢杆菌(Bacillus subtilis)Z54对番茄生长和根系细菌群落的影响。[方法]通过蘸根和灌根处理,分别将2株促生菌接种到基质栽培的番茄上。通过对番茄苗期各生长指标调查,明确促生菌对番茄生长的影响。利用16S rRNA基因扩增子测序技术,分析接种促生菌对番茄根系细菌群落组成和结构的影响。[结果]2株促生菌均能促进番茄生长:菌株JP2-3处理组的4种生长指标(株高、茎粗、最大叶长、最大叶宽)均比对照组大;除茎粗外,菌株Z54处理组其余3种指标均大于对照组。接种菌株JP2-3和Z54改变了番茄根系细菌群落的α多样性,丰富度指数和多样性指数都比对照组低,而且菌株Z54处理组降低更显著(P<0.05)。接种菌株254使根系细菌群落的β多样性发生了明显变化,而菌株JP2-3对β多样性的影响相对较小。番茄根系细菌群落的主要优势菌门有变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteriodetes);主要优势菌属有:链霉菌属(Streptomyces)、假单胞菌属(Pseudomonas)、艾德昂菌属(Ideonella)和德沃斯氏菌属(Devosia)等。在门水平上,基质栽培的番茄根系细菌群落组成与土壤栽培相似,但在属水平上有较大差异。接种菌株JP2-3提高了艾德昂菌属(Ideonella)、游动放线菌属(Actinoplanes)和水居菌属(Aquincola)等菌属的相对丰度;接种菌株Z54则提高了短波单胞菌属(Brevundimonas)和黄杆菌属(Flavobacterium)等菌属的相对丰度。在上述相对丰度增加的菌属中,有多种细菌是植物益生菌。基于线性判别效应量分析的结果表明,菌株JP2-3处理组的生物标志物主要集中分布于β-变形菌纲(Betapr[Background]Plant growth-promoting agents have been widely used in agricultural production,while the knowledge to their impacts on the root microbial community of crops in substrate culture is limited.[Objective]The objective is to investigate the effects of Pseudomonas sp.JP2-3 and Bacillus subtilis Z54 on the growth and root bacterial community of tomato in substrate culture.[Methods]The two strains were inoculated by root dipping and root irrigation for the tomato plants in substrate culture.We then measured the growth indexes of tomato seedlings to explore the effect of growth promoting bacteria on tomato growth.The strain effects on the composition and structure of the root bacterial community were analyzed by 16 S rRNA gene amplicon sequencing technology.[Results]The two strains promoted tomato growth.Four growth indexes(plant height,stem diameter,maximum leaf length,and maximum leaf width)in strain JP2-3 group were higher than those in CK group.Except stem diameter,the other three indexes in strain Z54 group were higher than those in CK group.Both strains JP2-3 and Z54 changed theαdiversity of bacterial community and decreased the bacterial richness and diversity compared with the CK group.Particularly,the decrease was more significant in strain Z54 group(P<0.05).Theβdiversity of root bacterial community was significantly changed by strain Z54 while slightly affected by strain JP2-3.The dominant bacterial phyla included Proteobacteria,Actinobacteria,Firmicutes,and Bacteriodetes,and the dominant genera were Streptomyces,Pseudomonas,Ideonella,Devosia,etc.The composition of root bacterial community of tomato in substrate culture was similar to that in soil culture at the phylum level while different at the genus level.Inoculation with strain JP2-3 increased the relative abundance of Ideonella,Actinoplanes,and Aquincola,while strain Z54 increased the relative abundance of Brevundimonas and Flavobacterium.Among the genera with increased relative abundance,many bacteria are beneficial to plants.LEfSe showed th

关 键 词：促生菌 高通量测序 基质栽培 微生物群落 

分 类 号：S641.2[农业科学—蔬菜学] S154.3[农业科学—园艺学]