Treatment of acute lung injury in mice using Bai-Ri-Ke syrup  

作　　者：Yue-Jie Yang Cui Zhang Meng-Meng Cui Yan-Mei Zong Li Wang Ying Li 

机构地区：[1]Department of Infectious Diseases,Tianjin Second People's Hospital,Tianjin 300192,China [2]Department of Pharmacy,Tianjin Second People's Hospital,Tianjin 300192,China

出　　处：《TMR Pharmacology Research》2022年第1期13-16,共4页TMR药理学研究

基　　金：supported by the Study on the mechanism of BRK in the treatment of pertussis based on"exterior and interior of lung and large intestine",Scientific research project of traditional Chinese medicine and integrated traditional Chinese and Western medicine of Tianjin Health Commission and Tianjin Administr。

摘　　要：Objective:This study aimed to examine the therapeutic effects of Bai-Ri-Ke syrup(BRK)on mice with acute lung injury(ALI).Methods:Fifty male C57BL/6 mice were equally divided into the control group,model group,dexamethasone(DXM)group,Bai-Ri-Ke syrup(BRK)low-group,and BRK-high group,with six mice per group.An intratracheal injection of 5 mg/kg POLY(I:C)was used to construct an ALI mouse model.After a successful model construction,the mice in the DXM group were given[0.2 mg/10 g·d]dexamethasone sodium phosphate injection(1 mL:2 mg)on the following day via intraperitoneal injection.The mice in the BRK-low group were given 0.015 mL APS everyday by gavage,and the mice in the BRK-high group were given 0.030 mL APS everyday by gavage for three days.The wet to dry weight(W/D)ratio of the lungs was observed every day.Bronchoalveolar lavage fluids(BALFs)were collected from the left lungs to measure the BALF protein level and neutrophil count after 72 h of treatment.The IL-6,IL-1β,and TNF-αlevels in BALF were also measured.HE staining was done to observe the histopathological changes in the lungs.Results:The ALI mice in the BRK-high group had significantly increased W/D(P<0.01).ELISA results showed that the DXM group and BRK-high group had significantly decreased BALF protein content(P<0.01),neutrophil count(P<0.01),and IL-6,IL-1β,and TNF-αlevels(P<0.01).Hematoxylin and eosin(H&E)results showed that the DXM group and BRK-high group had alleviated alveolar tissue injury,edema,bleeding,and inflammation.Conclusions:The BRK can decrease the W/D,BALF protein content,neutrophil count,and TNF-α,IL-1β,and IL-6 levels and alleviate the histopathological changes in the lungs of ALI mice.

关 键 词：pertussis acute lung injury inflammation bronchoalveolar lavage fluids 

分 类 号：R56[医药卫生—呼吸系统]