MALDI-TOF MS基质HCCA与含巯基生命物质加成反应的质谱研究  

Mass Spectrometric Studies on the Addition Reaction of HCCA and Sulfhydryl-Containing Living Substance in MALDI-TOF MS Analysis

作　　者：李静[1,2] 任其龙 潘远江[3] LI Jing;REN Qi-long;PAN Yuan-jiang(Key Laboratory of Biomass Chemical Engineering of Ministry of Education,College of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310027,China;Institute of Zhejiang University-Quzhou,Quzhou 324000,China;Department of Chemistry,Zhejiang University,Hangzhou 310027,China)

机构地区：[1]浙江大学化学工程与生物工程学院,生物质化工教育部重点实验室,浙江杭州310027 [2]浙江大学衢州研究院,浙江衢州324000 [3]浙江大学化学系,浙江杭州310027

出　　处：《质谱学报》2022年第2期133-141,I0001,共10页Journal of Chinese Mass Spectrometry Society

基　　金：浙江大学实验技术研究项目(SJS202008);浙江大学衢州研究院科技计划项目(IZQ2020KJ2013)。

摘　　要：采用基质辅助激光解吸飞行时间质谱(MALDI-TOF MS)和电喷雾质谱(ESI-MS)研究含巯基的生命物质(氨基酸、肽及蛋白质)与MALDI常用基质α-氰基-4-羟基肉桂酸(HCCA)之间的加成反应,并分别考察了其在酸性、弱酸性和碱性条件下的反应情况。结果表明,在碱性条件下,含巯基的生命物质易与HCCA发生加成反应,使待测物在质谱图中产生189.0 u的质量数迁移。此外,使用高分辨质谱分析加成产物,并通过串联质谱分析进一步确认其为共价结合产物。此研究为确定MALDI基质与含巯基的生命物质之间的加成反应提供了依据,以期减少质谱中基质对含有巯基化合物测试的干扰,为基质的选择提供参考。The addition reactions between the common matrixα-cyano-4-hydroxy cinnamic acid(HCCA)in matrix assisted laser desorption(MALDI)and the sulfhydryl-contained compounds were investigated using matrix assisted laser desorption time of flight mass spectrometry(MALDI-TOF MS)and electrospray mass spectrometry(ESI-MS),and the corresponding reactivity was also compared in the acid,weak acid and alkaline conditions,respectively.The results showed that the sulfhydryl-contained compounds could much easily undergo addition reaction with HCCA under alkaline conditions,resulting in a mass shift of 189.0 u in the mass spectrum.In addition,the identity of the reaction product was supported by high resolution mass spectrometry analysis,and was further identified as covalent bonding products by tandem MS analysis.This work provides a proof for the addition reaction between MALDI matrix and sulfhydryl-contained compounds,so as to reduce the interference of the matrix in the MALDI-TOF MS analysis of the relevant compounds,and to provide a reference for the selection of matrix.

关 键 词：基质辅助激光解吸飞行时间质谱(MALDI-TOF MS) 电喷雾质谱(ESI-MS) α-氰基-4-羟基肉桂酸(HCCA) 分析物-基质加合产物 含巯基生命物质 碎裂机理 

分 类 号：O657.63[理学—分析化学]